Avaliação da expressão salivar e tecidual das citocinas TGF-β e IL-10 em pacientes com carcinoma espinocelular de cavidade oral
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Data
2015-03-02
Autores
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Universidade Federal de Goiás
Resumo
The transforming growth factor β (TGF-β) and interleukin 10 (IL-10) are
immunosuppressive cytokines which promote failure of the local anti-tumor immune
response and, therefore, influence the proliferation and prognosis of malignant neoplasms.
The aim of this study was to investigate the tissue and salivary expression of TGF-β and
IL-10 in patients with oral squamous cell carcinoma (OSCC) and compare it with that of
healthy subjects (Control). The association of these cytokines with clinical parameters of
prognosis (staging, metastasis and survival) and histological grade of malignancy (WHO
grading) was also investigated. Cytokines in the tissue (OSCC, n = 65; Control, n = 30)
were identified using the immunohistochemistry technique (IHC) and in the saliva (OSCC,
n = 22; Control, n = 23) the Enzyme-linked Immunosorbent Assay (ELISA) was used. The
tissue expression of TGF-β and IL-10 in metastatic lymph nodes (n = 23) of OSCC
patients was investigated. The expression of TGF-β and IL-10 in the tissue was measured
using a semi-quantitative method in conjunction with staining intensity. Our findings
demonstrated a high tissue expression of IL-10 and TGF-β2, and a low or absent
expression of TGF-β1, in the majority of OSCC samples when compared to the group with
clinically healthy oral mucosa (Control) (p < 0.05 for IL-10 and TGF-β2). The salivary
concentration of IL-10 was also high, and distinguished the OSCC patients from their
healthy counterparts (p = 0.04), while the salivary concentration of TGF-β1 was similar for
both the OSCC and control groups (p = 0.97). The relationship between the cytokine
expression and clinical and microscopic prognostic factors showed that the expression of
IL-10 and TGF-β2 in neoplastic cells of the primary tumor was maintained by the
metastatic neoplastic cells in the cervical lymph nodes. The expression of TGF-β1
remained low or absent in the metastatic lymph nodes. It was shown that there was an
association between the high expression of IL-10 by tumor cells and the advanced clinical
stages (T3-T4) of patients (p = 0.02). Although not statistically significant, the expression
of TGF-β2 was higher in tumors at more advanced stages (p > 0.05). These findings
demonstrate that OSCC provides an immunosuppressive environment conducive to tumor
proliferation, with high expression of IL-10 and TGF-β2, which contributes to a worse
clinical prognosis. In addition, of the immunosuppressive cytokines investigated, IL-10 has
greater potential for becoming salivary biomarker when associated with an unfavorable
clinical prognosis of OSCC patients.
Descrição
Palavras-chave
Neoplasia bucal , IL-10 , TGF-β , Metástase , Saliva , Evasão Tumoral , Oral neoplasm , IL-10 , TGF-β , Metastasis , Saliva , Tumor Evasion
Citação
ARANTES, D. A. C. Avaliação da expressão salivar e tecidual das citocinas TGF-β e IL-10 em pacientes com carcinoma espinocelular de cavidade oral. 2015. 109 f. Dissertação (Mestrado em Odontologia) - Universidade Federal de Goiás, Goiânia, 2015.