Obesidade e estresse crônico em fêmeas de ratos wistar: avaliação comportamental, bioquímica e hematológica
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2015-08-17
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Universidade Federal de Goiás
Resumo
Obesity and chronic stress have been considered major public health problems that affect
millions of people worldwide. The objective of this study was to analyze the effect of obesity
associated with chronic stress on neurobehavioral parameters, hematological and biochemical
in rats, considering that the association of these diseases can enhance their negative effects on
homeostasis of the organism. For this, female Wistar rats were distributed in the following
groups: standard diet (SD), standard diet + stress (DP + stress), cafeteria diet (Cafe) and
cafeteria diet + stress (Cafe + stress) groups (n=12 rats, per group). The animals of the DP and
DP + stress groups were fed standard food. Groups Cafe and Cafe + stress, additionally to the
standard feed, were offered palatable and calorie-rich processed food, water with sucrose (300
g/L)and cola-type soft drink ad libitum. From the eighth experimental week, groups Std +
stress and Cafe + stress were subjected to restraint chronic stress model (50 days). After the
restraint chronic stress the animals were subjected to predictive anxiety tests (open field and
elevated plus maze tests) and depression (forced swimming test). At the end of the experiment
was evaluated hematological parameters (erythrogram, leucogram and platelet parameters)
and biochemical (lipid profile, blood glucose, total proteins and fractions). The results
demonstrate that cafeteria diet was able to induce obesity in the above animals, increasing
body mass, weight of retroperitoneal and visceral fat, body mass index, specific rate of weight
gain, weight delta efficacy coefficient food, weight gain coefficient for calorie intake, nasoanal
length, abdominal circumference and kilocalories ingested. The ratio locomotion in the
central quadrants/total locomotion evaluated during the open field test was not indicative of
anxiogenic or anxiolytic effect in the animal’s behavior. However, the elevated plus maze test
showed that obese and stressed animals were prone to higher anxiety levels. In addition, the
obese and stressed animals display less climbing behavior than all the other groups, which can
be considered an indicator of depression-like behavior. No damaging effects caused by the
interaction of obesity and stress were observed on the biochemical and hematological
parameters. Only individual effects of the factor obesity on leukocyte and glycemic
parameters were observed. The obese animals presented hypoglycemia and leukocytosis by
neutrophilia and lymphocytosis.
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ESTRELA, D. C. Obesidade e estresse crônico em fêmeas de ratos wistar: avaliação comportamental, bioquímica e hematológica. 2015. 89 f. Dissertação (Mestrado em Biodiversidade Animal) - Universidade Federal de Goiás, Goiânia, 2015.