Efeitos da ação bioestimulatória do Laser de baixa potência associada à aplicação de fração estromal vascular contendo células-tronco derivadas de tecido adiposo no tratamento de feridas por queimadura em ratos
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2015-05-22
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Universidade Federal de Goiás
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Stromal vascular fraction and irradiation with low level laser are resources potencially applicable in the treatment of burn injuries. Objectives This study aimed to: (i) analyze the macroscopic, microscopic and morphometric effects of stromal vascular fraction application compared to conventional care in 3rd degree burn injuries in rats; (ii) analyze the macroscopic, microscopic and morphometric effects of stromal vascular fraction containing adipose derived stem cells application associated with low level laser compared to conventional care in 3rd degree burn injuries in rats; (iii) whether the macroscopic, microscopic and morphometric effects of stromal vascular fraction containing adipose derived stem cells application associated with low level laser irradiation are dependent on the irradiation dose applied. Method: This project was approved by the Ethics Committee on the use of animals at UFG, protocol number 035/2013. All animals received injections of stromal vascular fraction prepared from adipose tissue extracted from a animal donor and occlusive dressing. The animals were divided into 5 groups: control (CT), stromal vascular fraction (SVF), stromal vascular fraction plus Laser at 27mW of power (SVFL27), stromal vascular fraction plus Laser at 53mW of power (SVFL53) and stromal vascular fraction plus Laser at 76mW of power (SVFL76).The animals were followed ove days 3, 7 and 30 for the evaluation of macroscopic, microscopic and morphometric aspects. For statistical analysis we used the parametric "t test". Results: The morphometric analysis showed a reduction in wound area in SVFL27 group compared to the others, but without statistically significant difference. In SVFL76 group was observed inhibition of wound contraction compared to SVFL27 and CT groups (p <0,05). Was observed on day 3 significantly increased (p <0.05) infiltration of polymorphonuclear, fibrin formation and in the number of fibroblasts in all treated groups compared to CT. On the 7th day the mononuclear infiltrate, angiogenesis, collagen and number of fibroblasts was significantly higher in all treated groups compared to CT (p <0.05). The SVFL27 group also showed a higher number of fibroblasts compared to groups SVF, SVFL53 and SVFL76. At 30 days significantly increased reepitalization and collagen deposition were observed in all treated groups compared to CT (p <0.05). The SVFL53 group also showed higher polymorphonuclear infiltrate compared to other groups (p <0.05). Conclusion: Isolated application of SFV or in combination with low level Laser gives better results than conventional care in burn injury in rats and the effects of the combination of the two features are dose
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LAMARO-CARDOSO, A. Efeitos da ação bioestimulatória do Laser de baixa potência associada à aplicação de fração estromal vascular contendo células-tronco derivadas de tecido adiposo no tratamento de feridas por queimadura em ratos. 2015. 86 f. Dissertação (Mestrado em Ciências da Saúde) - Universidade Federal de Goiás, Goiânia, 2015.