Transferibilidade e validação de marcadores microssatélites derivados de EST para duas espécies de Campomanesia (Myrtaceae) do Cerrado
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2014-06-02
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Universidade Federal de Goiás
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The Savanna is highly diverse and has the richest flora and endemic in the world, comprising about 12,000 species of native plants. The family Myrtaceae is among the 10 families representative of this biome, comprising 14 genera. Among these, gender Campomanesia has several fruit species, known popularly as gabiroba. The species Campomanesia adamantium and Campomanesia pubescens have widespread occurrence in the Savanna. Its fruits can be consumed " in nature ", in the form of candy, ice cream , soft drinks and as flavoring in alcoholic distillates, besides having antidiarrheal properties, its bark and leaves are used in the form of teas. His exploration is by extractivism and can also be grown in small family orchards, being a source of extra income for some families. Thus, the study of genetic diversity and genetic structure of populations’ allies with other areas of knowledge can provide important information for planning and execution of native species conservation programs information. Currently, molecular markers have wide application for studies on the genetics of populations, among the types of markers used; microsatellites have been widely used in recent years by high power to detect genetic variability. Population - genetic studies of the genus Campomanesia sp. are scarce and so not found microsatellite markers available in the literature for any species of the Savanna o. In this context, the aim of this study was to test the potential for transferability of microsatellite markers derived gene regions (EST) developed for Eucalyptus sp., Campomanesia in two species (C. adamantium and C. pubescens), providing a panel of polymorphic markers for the species. To do so, we sampled two populations of each species and DNA was extracted from leaf tissue. The potential for transferability of 120 primers pairs were evaluated. The PCR products derived from the microsatellite markers were assessed in automatic transferred DNA analyzer. The array of genotypes was used to estimate the parameters of genetic variability for the panel of marker. Of the 120 primers tested, 87 were discarded for not having amplification products, many nonspecific fragments amplified 22 and 12 were considered successfully transferred the two species. The 12 markers transferred showed good discrimination power of the individual , with high probability of paternity exclusion (0,99939 to C. adamantium and 0,99982 for C. pubescens) and low probability of identity (5,718 x 10-10 for C. adamantium and 1,182 x 10-11 to C. pubescens). The average number of alleles at polymorphic loci was equal to 6,8 to C. adamantium, ranging from 2 (EMBRA1335 and EMBRA 1811) 16 (EMBRA 1364) and C. pubescens was 7,8 ranging from 2 (EMBRA1811 and EMBRA 1076) to 16 (EMBRA 2011) alleles per locus. Mean genetic diversity of Nei (1973) in populations was equal to 0,517 (C. adamantium) and 0,579 (C. pubescens). Mean observed heterozygosity (Ho) for the populations was equal to 0,505 (C. adamantium) and 0,503 (C. pubescens). For some markers for deviations expected for the Hardy-Weinberg equilibrium was observed due proportion’s estimates of intrapopulation inbreeding (f) were not significant for any population. Inbreeding full level (F) was significantly different from zero, suggesting some genetic variability structure populations. The structure of genetic variability in component among populations (θp) was equal to 0,105 (C. adamantium) and 0,249 (C. pubescens).
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MIRANDA, E. A. G. C. Transferibilidade e validação de marcadores microssatélites derivados de EST para duas espécies de Campomanesia (Myrtaceae) do Cerrado. 2014. 103 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2014.