Utilização de porfirinas na inativação fotodinâmica do Herpesvírus bovino 1
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2016-02-29
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Universidade Federal de Goiás
Resumo
Bovine herpesvirus 1 (BoHV-1) is a virus of the family Herpesviridae and the
subfamily Alphaherpesvirinae, known to cause several diseases worldwide in cattle,
including rhinotracheitis, vaginitis, balanoposthitis, abortion, conjunctivitis, and enteritis. O
BoHV-1 is a source of economic loss for both dairy and beef producer due to a decrease in
dairy production, decrease in animal weight gain, a higher susceptibility to secondary
infections, and the occurrences of abortions. Aiming at new alternatives for the treatment and
disposal of these cattle pathogen, the photoinactivation becomes a viable alternative because
this treatment has shown to be effective against animal and human microorganisms. The
technique is based on the synergistic combination of a photosensitizer (PS), oxygen, and light.
When irradiated by a light source, the photosensitizer undergoes a series of photophysical
processes, which culminate in the generation of reactive oxygen species (ROS), which in
large amounts are toxic to pathogens. Aiming at a better efficiency of photoinactivation is
necessary to know the characteristics of each photosensitizer, analyzing the molecular
structure, quantum yield, lifetime, toxicity, stability, and absorption at specific wavelengths.
The irradiation time has shown to be very important because each PS reacts differently at a
given time. To evaluate the effect of the electrical charges present on PS, zinc insert ring in
the central ring of the molecule and the irradiation time in the photodynamic inactivation,
BoHV-1 aliquots were used with initial viral titer from 105.75 incubated for one hour with four
different photosensitizers: TPPS4, ZnTPPS4, TMPyP, and ZnTMPYP. Subsequently, they we
irradiated for two hours and sampled every 15 minutes. As control, we used virus without any
treatment, virus irradiated in the absence of PS, and virus with PS without radiating. After
irradiation, the sample was added to cultures of cells permissive to the virus, which was
analyzed for the presence of cytopathic effect (CPE), and the results were expressed as viral
titers. Although all treatments had significant differences, all PSs used in the study were
effective in the treatment of BoHV-1. The ZnTMPYP was the most efficient, fully inactivating
the virus with 30 minutes of irradiation, and the TPPS4 showed the worst result unable to
inactivate the virus after the period of irradiation, but considerably reduced the viral load of
BoHV -1. We also analyzed the photodegradation of a plasmid DNA using the same
photosensitizers. Again, ZnTMPYP showed better results.
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TELES, A. V. Utilização de porfirinas na inativação fotodinâmica do Herpesvírus bovino 1. 2016. 55 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2016.