Avaliação de fotossensibilizadores para a fotoinativação do Herpesvírus bovino I
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2016-02-29
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Universidade Federal de Goiás
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Photodynamic inactivation is a technique based on the synergistic combination of a photosensitizer, light and oxygen, resulting in the generation of reactive oxygen species, which are cytotoxic and cause cell death by apoptosis or necrosis. Bovine herpesvirus 1 is responsible for diseases of the respiratory and reproductive systems, it is estimated to occur an economic loss of a billion dollars a year, due to the action of the virus in the herd. In order to develop new alternatives and /or therapeutic approaches that are effective in viral clearance, we evaluated two photosensitizers, zinc phthalocyanine and aluminum phthalocyanine, the photoinactivation bovine herpesvirus 1. As a control we used irradiated virus without the presence of photosensitizer (light control) viruses incubated with photosensitizers without radiating (photosensitizer control) and viruses without any treatment. The light control showed that the use of light without the presence of photosensitizer does not have phototoxic effect on the virus; control showed that the photosensitizer zinc and aluminum phthalocyanines have no cytotoxicity in the absence of light. Both controls were compared to the virus that received no treatment. To better evaluate the irradiation time and the optimal concentration of the two photosensitizers, viral suspension aliquots containing 105.75 TCID50/mL were used, incubated with photosensitizers at concentrations of 5 and 10 μM and irradiated at 0, 15, 30, 45, 60, 75, and 90 minutes. After irradiation, the sample was added in permissive cell cultures virus, which was analyzed for the presence of cytopathic effect and the results were expressed as viral titers. The zinc phthalocyanine showed better efficiency in photoinactivation bovine herpesvirus 1, wherein the concentration of 10 μM with 30 minutes of irradiation was the most effective. The phthalocyanine aluminum inactivated virus completely after 60 minutes of irradiation, but there was no significant difference between concentrations. These results indicate that both phthalocyanines have a good applicability in photodynamic viral inactivation, but the zinc phthalocyanine has a better efficiency.
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OLIVEIRA, T. M. A. Avaliação de fotossensibilizadores para a fotoinativação do Herpesvírus bovino I. 2016. 37 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2016.