Avaliação do comportamento metabólico de cisticercos de Taenia crassiceps cultivados in vitro e expostos a condições estressantes
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Data
2013-09-06
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Universidade Federal de Goiás
Resumo
Taenia crassiceps cysticerci possess antigenic similarities to T. solium cysticerci and because of this and added to the relative easiness in its maintainance in laboratory they are used as an experimental model to T. solium studies.
The objective of this study was to analyze the in vitro influence of glucose and insulin on the energetic and respiratory metabolism of T. crassiceps cysticerci exposed to low dosages of anti-helminthic drugs, albendazol and praziquantel, and also to different concentrations of glucose and insulin. Glucose is the main energy source to these parasites in their larval and adult stages and glycogen is their main energetic reserve. Glycogen may be converted into glucose in case of low glucose uptake and is stored in the cysticerci tegument.
The cysticerci were collected from the peritoneal cavity of BALB/c female mice experimentally infected and maintained in the animal facilities of IPTSP/UFG. After 24hours of culture in RPMI culture medium supplemented and with glucose, glargine insulin (LANTUS®, Sanofi Aventis), albendazol and praziquantel, the cysticerci were removed and the medium was frozen in liquid nitrogen as to allow the metabolix stasis. Afterwards this culture medium was analyzes through HPLC as to permit the quantification of the following organic acids related to the carbohydrates metabolism: lactate and pyruvate, intermediary metabolism: citrate, α-ketoglutarate, succinate, fumarate, malate and oxaloacetate, fatty acids metabolism: β-hydroxibutyrate and propionate. It was possible to detect propionate and β-hydroxibutyrate secreted by T. crassiceps cysticerci which indicates the fatty acids oxidation as an alternative energy
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source used by the initial stage cysticerci which are in rapid growth. Also the detection of organic acids from the citric acid cycle indicates the in vitro aerobiosis performed by the initial stage cysticerci.
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Palavras-chave
Taenia crassiceps , Praziquantel , Albendazol , CLAE , Metabolismo , Avaliação in vitro , Insulina , Glicose , Ácidos orgânicos , Taenia crassiceps , Albendazole , Praziquantel , Metabolism , In vitro evaluation , Iinsulin , Glucose , Organic acids , HPLC
Citação
ANDRADE, L. C. M. Avaliação do comportamento metabólico de cisticercos de Taenia crassiceps cultivados in vitro e expostos a condições estressantes. 2013. 62 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2013.