A Influência do tempo de armazenamento, após a limpeza, na carga microbiana de tubos de silicone utilizados na assistência a pacientes cirúrgicos
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2016-04-12
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Universidade Federal de Goiás
Resumo
This experimental study examined silicone tubes coming from care to surgical patients in the
perioperative period, chosen randomly. The study was conducted from September to November
2015 and the tubes were derived from the Central Sterile Supply Departments (CSSD) of a large
general hospital in the Midwest region of Brazil. The objectives were to validate a method for
extraction and quantification of microbial contamination in silicon tubes, checking the microbial
charge of silicone tubes immediately after cleaning, and in different storage intervals identify the
presence of biofilms on silicone tubing.The study was approved by the Ethics Committee under
the protocols No. 1,277,077 and 1,000,946. To validate the method, two new sterile silicone tubing
were used, pipe 01 and pipe 02 to 106 were artificially contaminated with bacterial spores of
Geobacillus stearothermophilus. The contaminated pipes were filled with sterile water and sealed
at the extremities. The tube 02 was submitted to five minutes sonication. The tube 01 has not
submitted to this process. Subsequently, the seals were removed and the water collected in 60-
mL syringe and filtered through 0.45 Millipore membrane μm in holder for syringe device.
Membranes were incubated at 35 ° C for 24 hours in petri dishes containing nutrient agar. After
the incubation period, the membranes were removed and placed in test tubes containing 1mL of
saline that were submitted to vortexing for five minutes and subjected to handle calibrated
technique for quantification of the colony. The sonication proved to be more effective for recovery
of micro-organisms. The validated methodology was used for the tubes of the experimental groups
(consisting of 10 silicon tubes used in hospital care after the cleaning process), negative control
(three new silicone tubes) and positive control (tubes used in assisting during surgery with organic
matter visible). The tubes were initially segmented into three fragments: end 01, 02 and a half later
and were again targeted to pre-established time intervals zero, 12 and 24 hours in conditions
similar to those offered by the study institution. In addition to the groups submitted to
microbiological analysis by this method, similar tubes to the experimental group were collected for
analysis by scanning electron microscopy (SEM). Among the tubes that were subjected to analysis
by SEM, experimental groups were formed (three tubes used in hospital care after the cleaning
process), positive control (tubes used without cleaning) and negative (new tubes). There was no
statistically significant difference when comparing the means and the ends of the silicone tubing
used in hospital care (p> 0.05) in zero periods, 12 and 24 hours. There was an increase of
microbial load of the order of a magnitude on the logarithmic scale every 12 hours (p <0.05) in
cleaning and storage conditions provided by the institution in experimental and positive control.
There was no microbial growth in the negative control group. SEM showed the presence of organic
matter that can support microbial growth.
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TRINDADE, J. P. A. A Influência do tempo de armazenamento, após a limpeza, na carga microbiana de tubos de silicone utilizados na assistência a pacientes cirúrgicos. 2016. 96 f. Dissertação (Mestrado em Enfermagem) - Universidade Federal de Goiás, Goiânia, 2016.