Prevalência, perfil de suscetibilidade e caracterização molecular de Staphylococcus aureus isolados de uma linha de ônibus do sistema de transporte público coletivo do município de Goiânia-GO
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Data
2016-07-15
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Universidade Federal de Goiás
Resumo
Staphylococcus aureus is an important agent of health care and community infections.
Environmental surfaces, especially those in frequent contact with the hands, can serve as
reservoirs of microorganisms, working as fomites in microbial spread. In the public
transportation system, contact surfaces with hands can act as transmission vehicles,
assisting the dispersion of S. aureus in the community. The aim of this study was to
determine the prevalence of S. aureus and methicillin resistant S. aureus (MRSA) in
buses, terminals and platforms of a larger route of the public transportation system in the
city of Goiânia-GO, to determine the susceptibility and virulence profiles and assess the
genetic similarity among S. aureus isolates. We collected 852 swabs fromhandrails of
buses and ratchets of platforms and terminals that compose the East-West line. The
isolation of staphylococci was done by standard techniques and species identification by
detection of the femA gene. S. aureus identified were submitted to antimicrobial
susceptibility testing, conventional PCR for detection of mecA and lukS-Fgenes, multiplex
PCR to detect virulence factors genes, SCCmec typing and PFGE to assess the genetic
similarity among strains. The contamination prevalence of handrails and ratchets by S.
aureus was 17.7% (151/852). MRSA was detected in 0,5% of samples. The phenotype
known as iMLSB was detected in 41.0% (62/151) of the isolates. Several virulence
profiles were detected in 43 (28.5%)isolates, PFGE analysis revealed extensive genetic
diversity of circulating strains, including MRSA strains similar to USA300 and USA800.
The results suggest that S. aureus and MRSA strains, showing different susceptibility and
virulence profiles are circulating in the community. They may be transmitted between
individuals through fomites, putting the user population of the service at risk.
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Staphylococcus aureus , MRSA , Transporte coletivo , Virulência , MSSA , Fômites , Staphylococcus aureus , MRSA , Public transport , Virulence , MSSA , Fomites
Citação
NEVES, L. S. Prevalência, perfil de suscetibilidade e caracterização molecular de Staphylococcus aureus isolados de uma linha de ônibus do sistema de transporte público coletivo do município de Goiânia-GO. 2016. 120 f. Dissertação (Mestrado em Biologia das Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2016.