Indução de lacase de Pycnoporus sanguineus CCT 4518 induzida por resíduos agroindustriais e ensaio de biorremediação de estrogênio sintético (EE2)
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2016-04-25
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Universidade Federal de Goiás
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Laccase (EC 1.10.3.2, benzenediol: oxygen oxidoreductase) belongs to multicobre oxidases family, produced mainly by fungi. Due to its ability to catalyze oxidation of a wide variety of substrates, laccase has been used in various industrial applications such as delignification and bleaching of pulp, discoloration of dyes in textile effluents and detoxification of environmental pollutants. To meet the high demand of laccase at low cost is necessary to increase their production. One strategy is use of inducers such as metal ions and aromatic compounds. However, these compounds may have high toxicity and high cost. The use of agro-industrial and agricultural wastes containing laccase inducers is a sustainable and promising alternative strategy. The main of this study was to evaluate "in vitro" the capacity of ten agro-industrial wastes to induce the production of laccase from Pycnoporus sanguineus. We evaluated two different culture conditions (1% Malto extract and potato dextrose broth 0.5%) and the cultivation under stirring and static condition. The plant materials were added at a concentration of 1% (w / v) in 250 ml Erlenmeyer flasks containing 60 ml of culture medium. The best growing conditions have been established and the results of maximum laccase activity were with the medium CBD 0.5%, stirring 150 rpm at 28 ° C. Positive controls were performed (with induction xylidine and copper) and negative controls (no addition of inducer). All tests were performed in triplicate. The wastes that induced highest amount of enzyme were the cupuassu (1642U.mL-1) and cocoa (1589U.mL-1), guariroba´s straw (1258 U.mL-1) and açaí (886 U.mL-1), being greater than the induced positive control with 2,5-xylidine and copper (511 U.mL-1). Cupuassu and cocoa was selected for characterization. The enzymatic extract induced with cupuassu proved to be more stable at high temperatures and at different pHs, in addition to being able to oxidize different substrates. The extract was choice for the remediation of ethinylestradiol. Obtained 86% removal after 4 hours reaction and after 8 hours the concentration of hormone was below the method detection limit. Analysis of MS-ESI-TOF showed that hormone molecule was biodegraded during the enzymatic process.
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GOLVEIA, J. C. S. Indução de lacase de Pycnoporus sanguineus CCT 4518 induzida por resíduos agroindustriais e ensaio de biorremediação de estrogênio sintético (EE2). 2016. 83 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Federal de Goiás, Goiânia, 2016.