Identificação de metaloproteínas associadas a cobre, ferro e zinco codificadas pelos genomas de Paracoccidioides spp.
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2014-03-11
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Universidade Federal de Goiás
Resumo
Approximately one third of all proteins have been estimated to contain at least one metal cofactor, being
named metalloproteins. These represent one of the most diverse classes of proteins, containing metal ions
provide catalytic, regulatory and structural functions. Bioinformatic tools have been developed to predict
metalloproteins encoded by an organism based only on its genome sequence. Paracoccidioides complex
includes termodimorphic pathogenic fungi that are found as saprobiotic mycelia in the environment and as
yeast in host tissues. They are the etiologic agents of Paracoccidioidomycosis, a prevalent systemic mycosis
in Latin America. It is known that many metalloproteins are important for virulence of several pathogenic
microorganisms. On this way, the present work aimed to predict the cooper, iron and zinc proteins encoded
by the genomes of three phylogenetic species of Paracoccidioides spp. (Pb01, Pb03 and Pb18). Cu-, Fe- and
Zn-proteins represents 7% of the total proteins encoded by Paracoccidioides spp genomes. Zinc-proteins
were the most abundant metalloproteins representing 5.7% of the fungus proteome, while copper and iron
proteins represent 0.3% and 1.2% respectively. Functional classification revealed that metalloproteins are
related to many cellular processes. Furthermore it was observed that many of these metalloproteins play
roles as virulence factors, in the biology of the fungus, and an analysis of the protein interactions revealed
that many of them depend on each other to perform their functions. Thus, it is concluded that the Cu-, Feand
Zn- metalloproteome of Paracoccidioides is of utmost importance for biology and virulence
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Paracoccidioides , Genomas , Identificação , Bioinformática , Proteínas , Metais , Paracoccidioides , Genomes , Identification , Bioinformatics , Proteins , Metals
Citação
TRISTÃO, G. B. Identificação de metaloproteínas associadas a cobre, ferro e zinco codificadas pelos genomas de Paracoccidioides spp. 2014. 94 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2014.