Estadiamento, genótipos de HPV e metilação do gene WIF1 em câncer do colo do útero: associações com o prognóstico e sobrevida
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2016-10-14
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Universidade Federal de Goiás
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Introduction. Squamous cell carcinoma is the most common type of cervical cancer,
followed by endocervical adenocarcinoma. Tumor staging is important to evaluate
prognosis. Cervical infection by one of the oncogenic types of HPV, especially HPV 16
and 18 is a pre-requisite for developing invasive cancer. Epigenetics alterations, as
DNA methylation, are well-known carcinogenic mechanisms. WIF1 is a tumor
suppressor gene which silencing by methylation helps in neoplastic progression.
Objective. The goal of this study was to evaluate staging, HPV genotypes and WIF1
methylation in cervical cancer and to test association between these variables with age,
prognosis and survival. Methods. It was included 95 cases obtained in Araujo Jorge
Hospital (Goiânia/ GO): 73 of invasive squamous cell carcinoma and 22 of invasive
endocervical adenocarcinoma. DNA was extracted from paraffin-embedded biopsies
using phenol-chloroform. HPV detection and genotyping were conducted using the kit
INNO- LiPA HPV GENOTYPING EXTRA® (Innogenetics™). Methylation of WIF1
gene was evaluated by methylation-specific PCR (MSP). Odds Ratio was used to
calculate statistical association. The calculation of survival used the Kaplan-Meier
method and the log-hank test was used to compare means of survival and prognostic
factors. A value of p<0.05 was considered statically significant. Results. There was
significant association between tumor stages III and IV and worse prognosis (OR =
7.32). The 5-year overall survival was 79.1% and it was significant higher in cases with
tumor stages I and II (p = 0.001). Women between 50 and 60 years had more chances
having tumors in stages III and IV (OR = 0.15). Although, considering mean and
median ages of women included, those over 51 years were more likely having tumor
stages III and IV (OR = 5.92). No association was found between worse prognosis or
lower survival and histological type, HPV infection and WIF1 methylation. Conclusion.
Worse prognosis and lower survival was determined by tumor stages III and IV.
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CARVALHO, K. P. A. Estadiamento, genótipos de HPV e metilação do gene WIF1 em câncer do colo do útero: associações com o prognóstico e sobrevida. 2016. 91 f. Dissertação (Mestrado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás, Goiânia, 2016.