Avaliação do potencial genotóxico e antigenotóxico da fração aquosa e do isolado pedunculagina da semente de Myrciaria cauliflora (Mart) O. Berg em sistemas in vivo
Nenhuma Miniatura disponível
Data
2016-06-30
Autores
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Goiás
Resumo
Myrciaria cauliflora (Mart), commonly known as “Jabuticaba” (Brazilian Grape) occurs in all of
South America, especially in Brazil. This species presents medicinal properties and it is popularly
used to treat diarrhea and respiratory diseases. It has already been proven several biological
activities such as antioxidant, antimutagenic and anti-proliferative. The phytochemical analysis of
M. cauliflora detected the presence of several compounds, including the ellagitannin pedunculagin.
Due to the widespread use of this species for therapeutic and food purposes, this study aimed to
evaluate the genotoxic, antigenotoxic, cytotoxic and anticytotoxic actions of the aqueous fraction
(AF) and pedunculagin from M. cauliflora seed, by micronucleus (MN) test and the comet assay in
mice bone marrow cells. In order to analyze AF and pedunculagin activities prior to CP’s toxic
effects, the animals were co-, pre- and post-treated with these substances. The results showed that
AF at 24 h treatment did not show genotoxic or cytotoxic effects in the MN test and comet assay.
However, AF showed cytotoxic action, but did not show genotoxic effect at 120 h treatment in the
MN test. In the comet assay it was able to significantly reduce the frequency of DNA breaks when
compared to the negative control. In the evaluation of the antigenotoxic and anticytotoxic effects, it
was observed that in all treatments, in both assays, AF and pedunculagin showed antigenotoxic and
anticytotoxic activities, but in the post-treatment pedunculagin increased the cytotoxic effect of CP.
In general, our results indicated that AF and pedunculagin were able to protect the mice bone
marrow cells against DNA damage induced by CP in the MN test and comet assay. These findings
indicated that AF and pedunculagin could be probable candidates for the development of new
drugs.
Descrição
Citação
SILVA, R. M. Avaliação do potencial genotóxico e antigenotóxico da fração aquosa e do isolado pedunculagina da semente de Myrciaria cauliflora (Mart) O. Berg em sistemas in vivo. 2016. 33 f. Dissertação (Mestrado em Biodiversidade Vegetal) - Universidade Federal de Goiás, Goiânia, 2016.