Salmonella sp. em ovos e patos (Cairina moschata) de criações informais
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Data
2016-10-07
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Universidade Federal de Goiás
Resumo
The survey was conducted in order to investigate the presence of Salmonella sp. in swabs of
cloaca and duck eggs coming from 38 informal breeding sites and 38 markets, and to detect
anti-Salmonella Gallinarum and Pullorum antibodies in blood serum of these birds, in the
State of Goiás and the Federal Distrito. To determine the productive and sanitary profile of
the farms where ducks are raised, we applied 38 questionnaires consisting of 12 closed
questions. Through the information obtained by the responses to the questionnaires during the
visits, we observed that the farms of the studied regions did not present adequate productive
and sanitary management. We collected 324 whole blood samples were used in the test
Serological Rapid Agglutination plate (SAR) to detect anti-Salmonella pullorum antibodies.
In this test 117/324 (36.1%) blood sera were seropositive. We harvested 324 cloacal swabs
from ducks and 912 eggs from 38 farms and 38 markets. The samples were subdivided
according to the structure in pools, producing 228 eggshell samples, 228 albumen samples
and 228 yolk samples, totaling 684 samples. Cloacal swabs and eggs were analyzed by
conventional bacteriology for Salmonella sp. We obtained 38 isolates, 36 from eggs in 36/684
(5.3%), and the serovars were Salmonella Heidelberg, Schwarzengrund, antigenic form O:
9.12, and Salmonella typhimurium, and two isolates from cloaca swabs 02/324 (0.62%). The
identified serovars were Salmonella Typhimurium and Hadar. The 36 isolates from eggs and
two from cloacal swabs were submitted to susceptibility test to 15 antimicrobials, from which
we obtained the following distribution of resistance: 29/38 (76.3%) to sulfonomide, 14/38
(36, 9%) to enroflaxacin, 10/38 (26.3%) to amoxicillin, 7/38 (18.4%) to ampicillin, 7/38
(18.4%) to gentamicin, 6/38 (15.8%) to cotrimoxazole, 6/38 (15.8%) to cephalothin, 6/38
(15.8%) to trimethoprim, 03/38 (7.9%) to ceftiofur, 05/38 (13.1 %) to neomycin, 5/38
(13.1%) to tetracycline, 5/38 (13.1%) to fosfomycin, 3/38 (7.9%) to chloramphenicol, 2/38
(5.3%) to ciprofloxacin, and 0/38 (0%) to florfenicol. The percentage distribution of the
isolated serovars was: 32/38 (84.2%) Salmonella Schwarzengrund, 3/38 (7.9%) Salmonella
Typhimurium, 1/38 (2.6%) the antigen form: 9.12, 1/38 (2.6%) Salmonella Heidelberg, and
1/38 (2.6%) Salmonella Hadar. The 36 isolates of Salmonella were also submitted to proof of
real-time polymerase chain reaction (real-time PCR) on which the following genes were
detected: 33/35 (94.3%) for rfbJ, 07/35 (20%) for Int1 gene, and 06/35 (17,1%) for Sul1 gene.
Therefore, we verified circulation of Salmonella in ducks and their eggs in informal rearing
farms in the state of Goiás and in the Federal District, we also confirmed the antimicrobial
resistance and detected resistance genes. This information is important for determining risk
for both poultry and public health.
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Palavras-chave
Antimicrobianos , Genes , Propriedades , PCR , Resistência , Antimicrobials , Facilities , Genes , PCR , Resistance
Citação
DELFINO, D. A. A. Salmonella sp. em ovos e patos (Cairina moschata) de criações informais. 2016. 74 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2016.