Cartilagem auricular de bovinos tratada com solução alcalina: avaliação histológica pré- implantação e termográfica pós-implantação na parede abdominal de coelhos (Oryctolagus cuniculus)
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2017-02-06
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Universidade Federal de Goiás
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This study aimed to evaluate the viability of the decellularization process in bovine
cartilaginous tissue by histological analysis. For this purpose, 20 bovine elastic
cartilages were used, immersed in alkaline solution for 72 hours, followed by washing,
freezing and lyophilization of the material. Histological processing was then performed
to perform the analyzes. In the histological analyzes the decellularization and removal
of the amorphous substance from the tissue was observed, but the structure of elastic
fibers and collagen remained. It was concluded that the alkaline treatment was efficient
to obtain biomaterial from elastic cartilaginous tissue. This study also evaluated,
through thermographic examination, the feasibility of using implants with biomaterials
obtained from elastic cartilages treated in alkaline solution. The intensity of the
inflammatory process was observed through the variation of cutaneous temperature
comparing before and after the implantation of the biomaterials in rabbits (Oryctolagus
cuniculus). A total of 24 rabbits were evaluated, divided into two groups, of 12 animals
each, according to the implanted biomaterial. Evaluations were performed in the
preoperative period and after the implantation of biomaterials. In the (GI) group,
polypropylene mesh and elastic cartilage (GII) were treated with alkaline solution,
evaluated in the periods of seven and 35 days. From the results obtained, after
performing statistical tests, the group (GI) polypropylene mesh, obtained upper
abdominal skin temperatures, compared to the cartilage group, providing subsidies that
the use of elastic cartilage treated in alkaline solution, promoted lower inflammatory
intensity due to the processing to which it was submitted. It is concluded that treated
elastic cartilage has been shown to be a superior biomaterial relative to the mesh
polypropylene.
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FERREIRA, K. D. Cartilagem auricular de bovinos tratada com solução alcalina: avaliação histológica pré- implantação e termográfica pós-implantação na parede abdominal de coelhos (Oryctolagus cuniculus). 2017. 68 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2017.