Prospecção de marcadores químicos relacionados ao amarelecimento fatal em folhas de Elaeis guineensis utilizando abordagem metabolômica
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2017-03-03
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Universidade Federal de Goiás
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Oil palm (Elaeis guineensis) is the world’s major source of vegetable oil
and it has been able to adapt to the Brazilian subtropical area. Its growth,
largely done in Para, is affected by Fatal Yellowing (FY), a condition that can
cause plant’s death and has unknown cause. The aim for this master’s project
was to prospect and identify, using a metabolomics approach, possible
biomarkers of FY. To accomplish this study, for the first time different transport
and extraction methods were evaluated in order to verify the metabolic profile
stability in this species. Significant differences were noted and described using
a metabolic fingerprinting approach, with ultra high precision liquid
chromatography – mass spectrometry (UHPLC-MS) as the chemical analysis
and ANOVA simultaneous component analysis (ASCA) as the multivariate
statistic tool. After this study, leaves with and without FY symptoms were
transported using the previously optimizes protocol, analyzed and five
potential biomarkers were identified and related to their metabolic pathways,
using PCA as the chemometric tool. Finally, for the first time, the use of
advanced analytical techniques as MALDI imaging, which has been
highlighted as a powerful tool for identification of chemical compounds present
on tissue surface with spatial localization, has been applied to oil palm leaves
in order to perform a metabolic screening and search for the previously
identified biomarkers. An analytical protocol was established for analysis with
and without matrix application, for a wide range mass coverage and one of the
possible biomarkers was detected using the matrix-free approach.
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RODRIGUES NETO, J. C. Prospecção de marcadores químicos relacionados ao amarelecimento fatal em folhas de Elaeis guineensis utilizando abordagem metabolômica. 2017. 75 f. Dissertação (Mestrado em Química) - Universidade Federal de Goiás, Goiânia, 2017.