Propriedades citotóxicas da Beta-Lapachona em células de osteossarcoma in vitro
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2017-03-09
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Universidade Federal de Goiás
Resumo
Osteosarcoma is the main primary bone tumor, with unfavorable prognosis, high mortality
and high incidence of metastases. The treatment of choice is the removal of the tumor
associated with combined chemotherapy, whose adverse effects allude to the increasing need
to develop new drugs. The plants constitute a large natural reserve of compounds that have
medicinal and therapeutic properties, such as lapachol and its derivative, β-lapachone. The
aim of this study was to evaluate the cytotoxic effects of β-lapachone in osteosarcoma cells
cultured in vitro. Cells were cultured and treated with β-lapachone at different concentrations
and times of exposure. Tripan blue exclusion, tetrazolium reduction and cell survival assay
methods were performed to evaluate the effects of the compound on the cells. Cells treated
with 0,1μM β-lapachone showed lower initial cytotoxicity in the 24h time, whereas those
submitted to 1,0μM showed less viability after 72h of treatment. Cytotoxicity increased as the
concentration and time of exposure increased. The lowest IC50 (0,148μM) was observed in
treated cells for 72h. Cell growth after treatment was lower in the 1.0μl group after 72h and
the highest cell growth was observed under a concentration of 0.1μl after 24h. There was no
difference between groups for cell proliferation after treatment, and the cell survival fraction
was lower after 72h of exposure. It was concluded that β-lapachone has cytotoxic effects on
osteosarcoma cells cultured in vitro.
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GABRIEL, G. H. Propriedades citotóxicas da Beta-Lapachona em células de osteossarcoma in vitro. 2017. 42 f.
Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2017.