Efeito antibacteriano dos sistemas Self-Ajusting File, XP-endo finisher e irrigação ultrassônica passiva sobre biofilme de Enterococcus faecalis
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2016-04-19
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Universidade Federal de Goiás
Resumo
Abstract: Objective: To evaluate the effectiveness of complementary protocols of
sanitization in the decontamination of infected root canals. Methods: Fifty uniradicular
maxilary anterior human teeth were selected. The crowns were removed and the lengths
standardized at 16 mm. The specimens were inoculated with Enterococcus faecalis and
incubated at 37 °C for sixty days. Thirty teeth were performed with BioRace instruments
until diameter corresponding to #60.02, and then complemented with Self-Adjusting File
(SAF); XP-endo Finisher (XPF) and passive ultrasonic irrigation (PUI). Ten samples were
used as positive control and 10 were not contaminated. Initial and final samples were
collected and incubated at 37 °C for a period of 48 hours. The bacterial growth was
analyzed in culture, determining the presence or absence of bacteria. The optical density
of the culture medium was interpreted by UV spectrophotometry. The specimens were
sectioned and prepared for evaluation in SEM. The images of the root surfaces were
analyzed and classified by scores according to the presence of debris. The Kruskal-Wallis
test was used for statistical analysis. The level of significance was 5%. Results: The
mean optical density (μm) of the sanification protocols showed bacterial reduction in all
groups. The experimental groups did not present statistically significant differences (p =
0.196). The analysis of SEM images revealed no significant difference (p = 0.414)
between the scores of the groups. Conclusion: Complementary sanitization protocols
reduced bacterial contamination.
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SOUSA, V. C. Efeito antibacteriano dos sistemas Self-Ajusting File, XP-endo finisher e irrigação ultrassônica passiva sobre biofilme de Enterococcus faecalis. 2016. 57 f. Dissertação (Mestrado em Ciências da Saúde) - Universidade Federal de Goiás, Goiânia, 2016