Estudo funcional do gene gluc31 que codifica uma β-1,3-glucanase da família GH16 de Trichoderma harzianum
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2017-04-28
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Universidade Federal de Goiás
Resumo
The genus Trichoderma includes species that have the ability to antagonize plant pathogens in a
complex process ranging from antibiosis, competition for nutrients, mycoparasitism and induction of
defense mechanisms in plants or a combination of these. Trichoderma species are known for their
ability to produce lytic enzymes such as exoglucanases, endoglucanases, chitinases, and proteases that
is essential for phytopathogen cell wall degradation. In the development and differentiation processes
of Trichoderma, β-glucanases contributes significantly to the morphogenetic-morphological process
that lead to the presence of β-glucans as the main component of fungi wall. In this work, we studied the
functional role of the gluc31 gene that encodes an endo β-1,3-glucanase of the GH16 family of
Trichoderma harzianum ALL42 through the deletion of this gene. This study demonstrated that the
absence of Δgluc31 gene did not affect the in vivo mycoparasitism ability of the mutant T. harzianum
ALL42, however the involvement of this gene in cell wall remodeling and synthesis were
demonstrated. In the absence of the gluc31 gene, a higher deposition of chitin polymers on the cell wall
of the mutant hyphae was observed. The absence of the gluc31 gene in T. harzianum also demonstrated
an effect on the expression of other genes belonging to the family 16 of glycosyl hydrolases, due to the
function redundancy found among the glucanases.
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RIBEIRO, M. S. Estudo funcional do gene gluc31 que codifica uma β-1,3-glucanase da família GH16 de Trichoderma harzianum. 2017. 94 f. Tese (Doutorado em Biologia) - Universidade Federal de Goiás, Goiânia, 2017.