Efeitos do extrato padronizado de jabuticaba (Myrciaria cauliflora) sobre o sistema cardiovascular de ratos hipertensos
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2017-05-26
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Universidade Federal de Goiás
Resumo
The Brazilian flora presents many species of medicinal interest and the jabuticaba (Myrciaria cauliflora Berg) has occupied a favorable place in this segment by presenting several biological properties. Despite the important biological effects of jabuticaba, its actions on the cardiovascular system have not been clarified. Hence, it was hypothesized that jabuticaba hydroalcoholic extract (JHE) could improve the cardiovascular damages caused by hypertension. In this work, the hypertension was induced in male Wistar rats (200-230g, n=6 in all groups) by oral administration with L-NAME (60 mg/Kg/day in drinking water) for six weeks. JHE was administered orally (100 or 300 mg/Kg/day by gavage) starting at the second week along with oral treatment with L-NAME until ending of sixth week. The control group received only drinking water. The blood pressure and heart rate were recorded every week by tail-cuff plethysmography. After six weeks, the rats were anesthetized, sacrificed and the heart and left kidney were weight. Aortas were isolated and set up to isometric recordings in an organ bath to order to verify the capacity of contraction and relaxation. The capacity of JHE induce nitric oxide production in endothelial cells was analyzed by flow cytometry. Moreover, the phytochemistry composition and antioxidant potential of JHE were analyzed by HPLC, mass spectrometry and differential pulse voltammetry. Our phytochemistry and antioxidant results have shown the presence of phenolic compounds and antioxidant capacity to JHE. The arterial hypertension (225.8 ± 11.2 mmHg) was reduced after JHE 100 e 300 mg/kg/dia treatment (163.4 ± 8.7 e 172.1 ± 9.3 mmHg, respectively). The heart and kidney weight from hypertensive rats also were reduced. The vascular relaxation to acetylcholine and sodium nitroprusside and the contraction induced by phenylephrine were impaired in hypertensive group. The treatment with either 100 or 300 mg/Kg JHE significantly improved the vascular response for these agents. In conclusion, JHE presents high antioxidant potential. The treatment with JHE attenuated hypertension possibly improving the NO biodisponibility. The relaxation endothelium-dependant and independent was impaired by hypertension and improved after treatment with JHE.
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Jabuticaba , Hipertensão , L-name , NO , Aorta , Relaxamento vascular , Hypertension , Aorta , Vascular relaxation
Citação
SOUZA, C. G. Efeitos do extrato padronizado de jabuticaba (Myrciaria cauliflora) sobre o sistema cardiovascular de ratos hipertensos. 2017. 70 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Federal de Goiás, Goiânia, 2017.