Análise da tolerância á resistência em Trichoderma harzianum
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2017-07-31
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Universidade Federal de Goiás
Resumo
The presence of heavy metals in the soil has increased, causing many problems of
an agricultural nature. Trichoderma are a species which is among the most well know
and used biocontrol agents wordwide, as well as possessing qualities such tha
parasitism and antibiosis, they have great resistance to metals and can also absorb
them. Therefore, it is necessary for more detailed studies of the resistance of this
fungus to metals and to research further the processes involved. In this work, we
aimed to better understand the tolerance of T. harzianum to aluminum chloride,
though a series of experiments performed in BDA, MEX e MYG, as well Bradford
tests for quantification of proteins. In addition, we evaluated the gene response to
metal stress through the sequencing RNA samples obtained when the fungus was
created in BDA medium and submitted to concentrations of 1.5 and 3.0 mg / mL of
aluminum chloride, 1.5 mg / mL, the fungus showed significant inhibition. It was
observed that T. harzianum showed higher mycelial growth when grown in BDA
medium compared to MEX and MYG, presenting levels of protein secretion inversely
proportional to increasing concentrations of aluminum. The amount of transcriptionspecific
factors of the stress response was increased, as well as the induction of
genes involved in G-mediated cell signaling and increase in genes involved in
vacuolar transport and carrier proteins. A repression of genes encoding proteins
associated with cellular processes important for the growth of T. harzianum was also
observed. These results show that T. harzianum is resistant to aluminum since it is
able to tolerate and absorb large concentrations of this metal, which is commonly
harmful to agriculture.
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SANTOS, K. R. A. Análise da tolerância á resistência em Trichoderma harzianum. 2017. 60 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Goiás, Goiânia, 2017.