Estudo das atividades biológicas de frações proteicas do feijão comum (Phaseolus vulgaris) cultivar pérola
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Data
2017-06-30
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Universidade Federal de Goiás
Resumo
Common bean (Phaseolus vulgaris) is considered a nutraceutical food, because it has bioactive compounds, such as peptides. These peptides have several biological activities, such as chelating, antioxidant, anticancer, antihypertensive and other activities. Thus, this study aimed to assess the bioactivity of protein fractions of common bean (Phaseolus Vulgaris) cultivar Pérola. In this work the flour obtained from the peeled beans freshly harvested beans, hardened and hardened autoclaved were evaluated as a source of bioactive peptides. Four solutions were used for the production of extracts: acetonitrile/water/formic acid, sodium acetate, Ammonium bicarbonate and alkaline pH 8.0 solubilization. In the extracts and the protein fractions were measured the protein content, antioxidant activity tests with DPPH (2.2-diphenyl-1-picrilhidrazil), chelating activity of iron and copper. 1 extract was chosen to give the segment next steps for presenting greater bioactivity, thus he was submitted to fractionation by ultrafiltration in 10 kDa membrane, where verified your bioactivity, resistance to thermal treatment and the stability of the Chelation complex formed from iron and peptide gastric conditions. And was rated the content of antinutritional factors in the extracts submitted to heat treatment. These tests found that the bean plant presented greater biological activities tested than other beans. In tests with the protein fractions found that the peptides present bioactivity and are and resistant to heat treatment, and to form complex is resistant gastric conditions chelators. Observed that the thermal treatment reduces the amount of antinutritional factors. In vivo studies showed that despite being the iron complex-peptide to be stable gastric conditions, the system was not able to pass through the membrane of enterocytes, being totally eliminated by the feces. This work contributed to expand the study of bioactive compounds present in wastes from agro-industry, in order to guide the proper use and the best way to explore the nutraceutical potential of this legume.
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Feijão , Bioatividade , Atividade quelante , Autoclavagem , Absorção , Bioactivity , Stability , Autoclaving , Hardened, , Absorption
Citação
RIBEIRO, J. V. V. Estudo das atividades biológicas de frações proteicas do feijão comum (Phaseolus vulgaris) cultivar pérola. 2017.97 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Goiás, Goiânia, 2017.