Desenvolvimento de teste rápido para detecção de Listeria monocytogenes em alimentos
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Data
2018-02-27
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Universidade Federal de Goiás
Resumo
Listeriosis is a bacterial infection caused by Listeria monocytogenes, a bacterium transmitted
mainly by the ingestion of contaminated food. It is the main responsible for food infections with
low morbidity rate, but when contamination occurs in immunosuppressed the mortality rates are
high, representing an important public health problem. Methods available for identification of the
presence of Listeria monocytogenes include isolation of the bacterium through conventional
culture, laborious, time-consuming and low sensitivity method, and usually complex and costly
molecular and / or immunological methods requiring trained personnel and equipment. Therefore,
the search for rapid, sensitive and specific methods for detecting the presence of Listeria
monocytogenes in foods is useful and necessary for infection prevention and great value to reduce
the mortality rate in the immunocompromised as well as to enable the epidemiological surveillance
of this disease. The objectives of the study were: To develop lateral flow immunochromatographic
test for the identification of L. monocytogenes in foods; To evaluate the performance of the lateral
flow immunochromatographic test using food samples naturally and artificially contaminated with
L. monocytogenes. As the detection agent, were impregnated glass fiber with anti-internalin-A
MAb-2D12 conjugated to colloidal gold. As a capture agent, were sensitized, on nitrocellulose
paper, the test line with polyclonal anti-internalin-B antibody, and in the control line with protein
A, to validate the activity of the detection agent. Inoculations of standard strains of Listeria
monocytogenes were used for evaluation of the Listeria detection in the proposed test. As a
negative control, we used strains of Listeria innocua, Enterobacter sp. and Bacillus cereus.
Positivity was obtained in prototypes of rapid tests sensitized with Polyclonal Anti-InlB. Positivity
was observed in samples containing culture medium and newly cultured bacteria, validating the
methodology used in the development of the test. The detection capacity of the test at D
concentration is 2x10 4 CFU / mL.
Descrição
Palavras-chave
Listeria, Imunocromatografia, Alimentos, Imunocromatography, Food, Listeria
Citação
SILVA FILHO, E. Desenvolvimento de teste rápido para detecção de Listeria monocytogenes em alimentos. 2018. 99 f. Dissertação (Mestrado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás, Goiânia,
2018.