Desenvolvimento de novas metodologias PSI-MS para bioanálises
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2018-04-02
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Universidade Federal de Goiás
Resumo
Paper spray ionization (PSI) is an environmental ionization technique in mass spectrometry (MS), where the sample is
deposited on triangular paper moistened with solvent and the ionization process occurs by the application of high
voltage on the paper. An important factor that can affect its performance is ion suppression. Aiming to reduce this
effect and the exclusion of macromolecules in complex samples, the RAM-PSI-MS method for the analysis of
dopamine and epinephrine in plasma was developed and validated. A methodology for analyzing creatinine in the urine
was also developed using graphene oxide (GO) modified paper. The methods developed were efficient and promising
in improving the role for PSI-MS analysis. The graphene oxide coating was able to improve the time and stability of
the spray, in addition to reducing the effect of ion suppression. Already coating the paper with ISRP-RAM phase made
it possible to analyze a complex sample, such as plasma, without any sample preparation, obtaining very satisfactory
results. Showing that it is possible, practically, to eliminate the inherent problems of PSI-MS, only with the paper
coating.
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FERNANDES, A. R. Desenvolvimento de novas metodologias PSI-MS para bioanálises. 2018. 88 f. Dissertação (Mestrado em Química) - Universidade Federal de Goiás, Goiânia, 2018.