Isolamento e caracterização fenotípica e genotípica de Salmonella sp. em vísceras de frangos
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2017-08-31
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Universidade Federal de Goiás
Resumo
Salmonella sp. in food of poultry origin represents a major problem for world
public health. The viscera of broilers can also be contaminated with the microorganism being
the potential carrier of this pathogen. In this context, the objective of this work was to
investigate the occurrence of Salmonella sp. in edible and inedible viscera of broilers, the
presence of virulence genes (invA, and spvC) and resistance genes (sul1 and bla TEM ), as well
as susceptibility profile to antimicrobial bases. The samples were collected in two
slaughterhouses where 405 samples were obtained, 150 hearts, 150 livers and 105 biliary
vesicles. Of these, seven samples (1.73%) were positive. The serovars found were Salmonella
Minnesota and Salmonella Swarzengrund. Antimicrobial principles were tested for
susceptibility to antimicrobials. The highest percentages of resistance were observed for
gentamicin (100%), ciprofloxacin (100%), enrofloxacin (100%) and florfenicol (100%),
followed by ampicillin, doxycycline, tetracycline (71%) and amoxicillin (57%). All isolates
were sensitive to sulfamethoxazole + trimethoprim. The invA gene was identified in all
isolates. The The spvC gene was identified in 50% S. Minnesota. Regarding the resistance
genes, bla TEM was identified in all isolates. The sul1 gene was detected in four of the seven
isolates. From the results, S. Minnesota and S. Swarzengrund of avian origin have genes that
enable invasion, as well as phenotypically express resistance to nine antimicrobial bases
tested.
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COSTA, C. S. C. Isolamento e caracterização fenotípica e genotípica de Salmonella sp. em vísceras de frangos. 2017. 40 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2017.