Suscetibilidade a antimicrobianos e genes de virulência em Salmonella enterica de origem avícola
Nenhuma Miniatura disponível
Data
2016-12-22
Autores
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Goiás
Resumo
Salmonella enterica is a foodborne pathogen with multifactorial and complex pathogenic
mechanisms. Identification of the presence of virulence genes and antimicrobial resistance profiles
in isolates of poultry origin provides relevant information on the risk attributed to the consumption
of products contaminated by the agent. The objective of this study was to verify the susceptibility
profile of Salmonella enterica for nalidixic acid (30μg), amicacin (30μg), ampicillin (10mg),
ceftiofur (30μg), chloramphenicol (30μg), ciprofloxacin (5μg), enrofloxacin (5μg), streptomycin
(10mg), gentamicin (10mg), tetracycline (30μg), tobramycin (10mg) and trimethoprim (5μg) used
in both human and animal medicine, to investigate the presence of multiresistant isolates, to detect
the presence of the variable region of the class 1 Integron, to analyze the association between the
presence of Class 1 Integron and antimicrobial resistance and to evaluate the presence of virulence
genes located in the islands of virulence 1 (invA) and 2 (sseD), gene encoding long polar fimbriae
(lpfA) and plasmidial spvR, to identify the virulence profiles and pathogenicity potential of
Salmonella enterica serovars isolated from carcasses, hearts, livers, gizzards and environment of
slaughterhouses located in the State of Goiás and on chicken carcasses marketed in commercial
establishments in Goiânia -GO. The highest resistance frequency was observed for ceftiofur,
19.12% (13/68), followed by streptomycin, gentamicin, tobramycin, tetracycline and trimetropic,
16.18% (11/68) both, nalidixic acid 14.71% (10/68), ampicillin 13.24% (9/68), and enrofloxacin
2,94% (2/68). No resistance was observed for ciprofloxacin, only intermediate, 45.59% (31/68),
100% (68/68) of the isolates were sensitive to amikacin and chloramphenicol. Of the 68 isolates
22 (32.35%) were resistant to one or more antimicrobial principles. Twelve profiles of
antimicrobial resistance were identified, 54.54% (12/22) of the isolates presented multiresistance.
The variable region of Class 1 Integron was detected in 63.23% (43/68) of the isolates. The
presence of this region was not associated with antimicrobial resistance. All slaughterhouses and
in most commercial establishments it was possible to identify Salmonella enterica carrying the
Integron of class 1 demonstrating the ubiquity of the same. The invA gene was identified in 100%
(59/59), sseD in 92.53% (54/59), lpfA in 86.51% (52/54) and spvR in 86.18% (49/59) of the
serovars of Salmonella enterica. Six virulence profiles were identified, 77.97% of the isolates were
grouped in profile A characterized by the presence of the four virulence genes simultaneously. The
knowledge of the virulence profiles of the isolates allows to affirm that the serovars identified in
the state of Goiás are potentially virulent and capable of triggering disease in poultry production
systems and in humans.
Descrição
Palavras-chave
Citação
OLIVEIRA, A. P. Suscetibilidade a antimicrobianos e genes de virulência em Salmonella enterica de origem avícola. 2016. 73 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2016.