Nanopartículas poliméricas e interações com macrófagos
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Data
2016-09-16
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Universidade Federal de Goiás
Resumo
Nanotechnology involves the creation and use of materials, devices and systems through
control of matter on the manometer scale. It has positive impact on medicine involving the
treatment and diagnosis. Drug delivery systems prepared with nanostructures have the ability
to overcome biological barriers and optimizing drug release. However, one of the main
challenges in the use of these systems is their internalization by macrophages. This study
aims to prepare and characterize polymer nanoparticles formed by biodegradable polymers
and investigate its impact on the function of macrophages in vitro by the observation of cell
viability, phagocytic activity and cytokine production. Also, was investigate the cytokine
profile in serum and splenocytes culture supernatant from Balb/C mice injected with chitosan
nanoparticles. The chitosan and chitosan-containing magnetic nanoparticles were prepared by
ionic gelation crosslinking, resulting in a morphology "nearly spherical" and positive zeta
potential. The PLGA prepared by emulsification and solvent evaporation method presented
spherical morphology and negative zeta potential. All preparations had a diameter smaller
than 300 nanometers and polydispersity index lower than 0.5. Nanoparticles of chitosan and
PLGA affected the viability of macrophages in vitro only at the highest concentration tested (4
mg/mL). The chitosan nanoparticles containing magnetic nanoparticles were internalized by
macrophages after 4h and 24h of incubation. Different nanoparticles triggered high
production of TNF- and low IL12p40 production and IL-10. Serum and splenocytes culture
supernatant was observed in both the production of IFN- as IL-4. However, IL-4 production
was higher than that of IFN-.
Descrição
Palavras-chave
Nanopartículas , Quitosana , PLGA , Nanopartículas magnéticas , Macrófagos , MET , Fagocitose , Nanoparticles , Chitosan , PLGA , Magnetic nanoparticles , Macrophages , Phagocitose , TEM
Citação
BANDEIRA, A. C. Nanopartículas poliméricas e interações com macrófagos. 2016. 69 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2016.