Expressão imuno-histoquímica de células - tronco mesenquimais em lesões periapicais crônicas
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2019-03-07
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Universidade Federal de Goiás
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Introduction: Mechanisms of the pathogenesis process of chronic periapical lesions
still require further discussion. Mesenchymal stem cells (MTC) present in the periapical
region may participate in the process of genesis and development of chronic apical
periodontitis, due to the ability of migration and differentiation in inflammatory
environments. Objective: To evaluate the immunohistochemical expression of CTM
markers (CD44, CD45, CD73 and CD105) in chronic periapical lesions. Method: Ten
samples of periapical cysts and ten periapical granulomas were selected from the block
file of the endodontic science laboratory of the Faculty of Dentistry of the Federal
University of Goiás to perform an immunohistochemical reaction. The frequency of the
presence or absence of the CD44, CD45, CD73 and CD105 markers was assessed
under light microscopy in the fields of higher expression, and the immunohistochemical
expression of CTM was determined when there was positive co-expression on the CD44,
CD73 and CD105 markers and negative expression for the CD45 marker. The frequency
of the qualitative variables were obtained and evaluated by the Chi-square test. Values
of p <0.05 were considered significant. Results: In the periapical granulomas, 20%
(2/10) presented co-expression for CTM positive markers (CD44, CD73 and CD105)
associated with no expression for the CTM (CD45) negative marker. In the periapical
cysts group there were no cases (0/10) of immunohistochemical coexpression for CD44,
CD73 and CD105 markers associated with absence of CD45 expression. Conclusion:
In the periapical cysts there was no immunohistochemical expression of CTM and the
immunohistochemical expression occurred in 2 of the 10 cases of periapical granulomas.
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SOUZA, P. O. C. Expressão imuno-histoquímica de células - tronco mesenquimais em lesões periapicais crônicas. 2019. 46 f. Dissertação (Mestrado em Odontologia) - Universidade Federal de Goiás, Goiânia, 2019.