Comparação da prevalência de parasitos entéricos em gatos errantes e domiciliados em Goiânia-Goiás, análise da acurácia de técnicas parasitológicas e avaliação da copro-PCR para o diagnóstico de Toxoplasma gondii
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2016-12-13
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Universidade Federal de Goiás
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Cats are backers of zoonotic agents to man, including intestinal parasites, including Toxoplasma gondii. The aim of this study was to evaluate the prevalence of enteric parasites in stray cats and cats resident in the period from March 2015 to May 2016; analyzing the accuracy of various laboratory methods for diagnosis of enteric parasites and verify Copro-PCR performance in conventional parasitological confirmation of diagnosis and differential diagnosis of T. gondii oocysts. Samples of faeces were collected processed by methods Willis, Sheather, Faust and Hoffman-Janer-Pons-Lutz (HJPL) and Copro-PCR amplification using T. gondii B1 gene. The accuracy analysis was performed by determining the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and kappa (k). We collected 65 samples of feces of stray cats captured by the CCZ of Goiania-GO and a Non Governmental Organization protector of animals and 84 samples of resident cats in Goiânia-GO, with a total of 149 samples. The prevalence of enteric parasites in cats roving was 60% (39/65) and the resident cats was 17% (14/84). In positive samples of stray cats, it was observed that 43.5% (17/39) of the animals were infected by monoparasitism, 30.7% (12/39) had eggs of Hookworm; 5.1% (2/39) T. gondii/H. hammondi oocysts; 5.1% (2/39) Toxocara cati; 2.6% (1/39) oocysts Cystoisospora sp .; and 56.5% (22/39) of the animals had multiple parasitic infections, and 18% (7/39) were infected by Hookworm, Cystoisospora sp. and T. gondii/H. hammondi; 13% (5/39) T. gondii/H. hammondi and Cystoisospora sp.; 10.2% (4/39) T. gondii / H. hammondi and Hookworms; 10.2% (4/39) for Hookworms and Cystoisospora sp.; 5.1% (2/39) for Hookworms and T. cati. In the group of resident cats, monoparasitism was observed, and 57.1% (8/14) were infected by Hookworm; 21.4% (3/14) T. gondii/H. hammondi; 14.3% (2/14) T. cati; 7.2% (1/14) of Cystoisospora sp. The accuracy analysis for Hookworms and T. gondii / H. hammondi the Faust technique performed better, and Cystoisospora sp., the HPJL technique obtained higher accuracy. In Copro-PCR of the 23 samples identified as T. gondii/H. hammondi, 6 were diagnosed as T. gondii, demonstrating the ability to perform the technical differential diagnosis. In addition to Copro-PCR it was also possible to identify the presence of genetic material of T. gondii 16.1% (24/149) of the fecal samples analyzed. 12.1% (18/149) of the positive samples by Copro-PCR were negative in parasitological examinations of conventional stool. 4% (6/149) of the samples were positive agreement by conventional parasitological examinations and Copro-PCR. Comparing the two groups, it was observed that stray cats have a high prevalence of enteric parasites in relation to pet cats. Copro-PCR is a tool capable of performing the differential diagnosis of coccidia and its high sensitivity positive samples detected even considered negative by the gold standard. These results allow us to conclude that cats have epidemiological importance in environmental contamination and transmission of zoonoses, both stray cats, as the resident, who had high parasitism in this study.
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LIMA, Jaqueline Ataíde Silva. Comparação da prevalência de parasitos entéricos em gatos errantes e domiciliados em Goiânia-Goiás, análise da acurácia de técnicas parasitológicas e avaliação da copro-PCR para o diagnóstico de Toxoplasma gondii. 2016. 67 f. Dissertação (Mestrado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás, Goiânia, 2016.