Investigação dos efeitos do treinamento com ß-glucana no controle da infecção por Leishmania (Viannia) braziliensis em camundongos C57BL/6 transgênicos para interleucina 32ƴ humana
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2019-03-08
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Universidade Federal de Goiás
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B-glucan induces trained innate immunity in monocytes/macrophages. Studies suggest that IL-32 is involved in mecanisms important for the control of L. braziliensis and transgenic mouse for the human IL-32 gene (IL-32) is a model for evaluating the functions of this cytokine. Thus, this study aims to evaluate the effect of -glucan training on the control of Leishmania braziliensis infection in IL-32Tg or wild-type (WT) mice. The mice were trained with -glucan and infected with L. braziliensis in the paw (105 or 106 parasites). We evaluated: lesion size, parasite load, histopathological characteristics, cytokines in macerated of the infected paws, lymph node and bone marrow cell cultures. Macrophages were derived from bone marrow precursors from WT or IL-32Tg animals after training in vivo and infected with L. braziliensis to evaluate phagocytosis and leishmanicidal activity. In IL-32Tg mice infected with 105 parasites, when compared to WT animals, -glucan training led to an increase in lesion size on week 3 of infection, associated with an increased inflammatory process and increased production of interferon gamma (IFN); and less parasitic load and less intense inflammatory process after 8 weeks of infection. In bone marrow cell cultures of IL-32Tg animals, 7 days after -glucan injection, there was an increase in IL-1production after stimulation with Leishmania antigens. The phagocytic activity of macrophages from IL-32Tg animals trained with -glucan was higher than that of trained WT animals, but the ability to control infection was similar. Data with inoculum of 105 parasites suggest that IL-32 enhances the effects of
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FIGUEIREDO, A M. B. Investigação dos efeitos do treinamento com ß-glucana no controle da infecção por Leishmania (Viannia) braziliensis em camundongos C57BL/6 transgênicos para interleucina 32ƴ humana. 2019. 81 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2019.