Selective antibacterial activity of the cationic peptide PaDBS1R6 against Gram-negative bacteria
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Data
2019
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Resumo
Infections caused by Gram-negative bacteria, Escherichia coli and Pseudomonas aeruginosa foremost among them,
constitute a major worldwide health problem. Bioinformatics methodologies are being used to rationally design
new antimicrobial peptides, a potential alternative for treating these infections. One of the algorithms used to
develop antimicrobial peptides is the Joker, which was used to design the peptide PaDBS1R6. This study
evaluates the antibacterial activities of PaDBS1R6 in vitro and in vivo, characterizes the peptide interaction to
target membranes, and investigates the PaDBS1R6 structure in contact with mimetic vesicles. Moreover, we
demonstrate that PaDBS1R6 exhibits selective antimicrobial activity against Gram-negative bacteria. In the
presence of negatively charged and zwitterionic lipids the structural arrangement of PaDBS1R6 transits from
random coil to α-helix, as characterized by circular dichroism. The tertiary structure of PaDBS1R6 was determined by NMR in zwitterionic dodecylphosphocholine (DPC) micelles. In conclusion, PaDBS1R6 is a candidate for the treatment of nosocomial infections caused by Gram-negative bacteria, as template for producing
other antimicrobial agents.
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Antimicrobial peptide (AMP), PaDBS1R6, Escherichia coli, Pseudomonas aeruginosa, Antibiotic resistance, Drug design, Joker algorithm
Citação
FENSTERSEIFER, Isabel C. M. et al. Selective antibacterial activity of the cationic peptide PaDBS1R6 against Gram-negative bacteria. BBA: biomembranes, Amsterdam, v. 1861, n. 7, p. 1375-1387, 2019. DOI: 10.1016/j.bbamem.2019.03.016. Disponível em: https://www.sciencedirect.com/science/article/pii/S0005273619300744?via%3Dihub. Acesso em: 16 fev. 2024.