Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/12244
Tipo do documento: Artigo
Título: Association of TLR4 polymorphisms with subclinical mastitis in Brazilian holsteins
Autor: Mesquita, Adriano Queiroz de
Rezende, Cintia Silva Minafra e
Mesquita, Albenones José de
Jardim, Eurione Antonio Garcia da Veiga
Junqueira-Kipnis, Ana Paula
Abstract: The identification of dairy cows with greater or lower potential to develop mastits has been pursued for many years among different segments of the milk industry, including governmental organizations. Genomic studies have suggested that Single Nucleotide Polymorphisms (SNPs) within the pattern recognition receptors (PRR) could lead to different responses to pathogens, and consequently result in mastitis resistance or susceptibility. To investigate whether toll like receptor 4 (TLR4) gene is associated with subclinical mastitis in Holstein cows from a property in the state of Goiás, Brazil, TaqMan allelic discrimination and somatic cell count were performed. One hundred and fifty milk samples were analyzed for SCC and centesimal composition. Twenty percent of those samples with SCC above 200,000 (n=13) were screened for real-time PCR identification of microorganisms and blood samples were genotyped for TLR4 SNPs. There was a higher prevalence of Gram-positive bacteria in the analyzed samples (88.9%) and animals that had the combined genotypes AACCCC, GGTCGG and GACCGC presented the lowest somatic cell scores, and consequently those genotypes have the potential to be applied as molecular markers for assisted animal selection to improve milk quality.
Palavras-chave: TLR-4
Single nucleotide polymorphism
Mastitis
Milk quality
País: Brasil
Instituição: Sociedade Brasileira de Microbiologia
Unidade acadêmica: Escola de Veterinária e Zootecnia - EVZ (RG)
Citação: MESQUITA, Adriano Queiroz de; REZENDE, Cintia Silva Minafra e; MESQUITA, Albenones José de; JARDIM, Eurione Antonio Garcia da Veiga; KIPNIS, Ana Paula Junqueira. Association of TLR4 polymorphisms with subclinical mastitis in Brazilian holsteins. Brazilian Journal of Microbiology, São Paulo, v. 43, n. 2, p. 692-697, Apr./June 2012.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1590/S1517-83822012000200034
Identificador do documento: 10.1590/S1517-83822012000200034
URI: http://repositorio.bc.ufg.br/handle/ri/12244
Data de publicação: Jun-2012
Aparece nas coleções:EVZ - Artigos publicados em periódicos

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