Diagnóstico de Babesia bigemina por reação em cadeia da Polimerase (PCR) utilizando os primers GAU6 e GAU7 para um fragmento de SSrRNA

Resumo

The present study was carried out to evaluate the polymerase chain reaction (PCR) protocol with GAU6 and GAU7 primers for the SSrRNA of Babesia bigemina in cattle blood containg Babesia bigemina, Babesia bovis and Anaplasma marginale. Forty reactions were carried out for blood samples with haemo-parasites, 14 PCR for Babesia bigemina, 12 for Babesia bovis and 14 for Anaplasma marginale. DNA was extracted from the blood samples using the commercial GFX™ Pharmacia Biotechâ, and then the PCR protocol as described by LINHARES et al. (2000). The results show that the GAU6 and GAU7 primers are specific for Babesia bigemina and did not show a positive reaction for the Babesia bovis and Anaplasma marginale parasites. No reaction was obtained with DNA extracted from blood of healthy cattle.

Descrição

Palavras-chave

Babesia bigemina, Polimerase, PCR, Polymerase

Citação

SANTANA, A. P.; LINHARES, G. F. C.; BORGES, G. T.; MESQUITA, A. J.; MIRANDA, A. H.; MADRUGA, C. R.; MINHARRO, S. B. Diagnóstico de Babesia bigemina por reação em cadeia da Polimerase (PCR) utilizando os primers GAU6 e GAU7 para um fragmento de SSrRNA. ARS Veterinária, Jaboticabal, v. 17, n. 3, p. 224-229, 2001.