Eficiência do enriquecimento em duplo estágio realizado nos caldos UVm e fraser e desempenho dos ágares LPM e L.Sab-Can para a detecção de listuia spp. em carne de frango naturalmente contaminada
Carregando...
Data
1995-12
Título da Revista
ISSN da Revista
Título de Volume
Editor
Antônio Henrique Garcia
Resumo
lt was determined the performance of two selective broths and two agars to isola te listeria from chicken meat naturally contaminated, acquired in the supermarkets of Goiânia-Goiás, in the period between January and June 1992. Methodology of isolation involved the utilization of two-stage selective enrichment in the University of V ermon and Fraser broths and plating in lithiurn chloride-phenyletanol-moxalactam agar and the listeria selective agar base supplemented with cicloheximide, acriflavine and nalidixic acid. Incubation was done at 30ºC for 48 hours. Cultures of secondary enrichment broth were innoculated in duplicated plates with and without previous treatment with a 0,25% potassium hydroxide so!ution during one minute, while the plates from primary enrichment broth were innoculated only with treated cultures. Primary and secondary selective broths showedcomparable efficiency at the statistic point ofview (Z= -1,7393<1,96), identifying 90,28% and 97 ,22% of ali positive sarnples of this experiment, respectively. Using the lithiurn ch!oride-pheniletanol-moxalactarn agar and the Listeria selective agar base supplernented with cicloheximide, acriJJavine and nalidixic acid were detected 98,61 % and 54, 17% ofthe positive samples. These diferences were significant at statistic view. The treatrnent of broths with 0,25% potassiurn hydroxide showed a little reduction in the nurnber of positive sarnples detected.
Descrição
Palavras-chave
Listeria, Caldo de enriquecimento, Carne de frango, Enrichment broth, Chicken meat
Citação
NUNES, Iolanda Aparecida; ROSSI JÚNIOR, Oswaldo Durival; MESQUITA, Albenones José de; GARCIA, César Augusto. eficiência do enriquecimento em duplo estágio realizado nos caldos UVm e fraser e desempenho dos ágares LPM e L.Sab-Can para a detecção de listuia spp. em carne de frango naturalmente contaminada. Anais da Escola de Agronomia e Veterinária, Goiânia, v. 25, n. 2, p. 13-20, jan./dez. 1995. Disponível em: <https://www.revistas.ufg.br/pat/article/view/2895/2944>.