Comparison of two methods to quantify 2,6-Dichlorophenol from tick Amblyomma cajennense by GC/MS-SIM
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Data
2010
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Resumo
Amblyomma cajennense (Acari: Ixodidae) is a tick of socioeconomic importance in
the South American sub-continent. Nevertheless, little is known pertaining to its chemical
ecology, information deemed crucial to its management. In this study, the tick sex pheromone
2,6-dichlorophenol (2,6-DCP) was quantified from A. cajennense fed on rabbits for 6 days by use
of gas chromatography–mass spectrometry in the selected ion monitoring mode (GC/MS-SIM).
The sex pheromone extract was obtained by the exposure of attractive females to hexane and
ultrasound probe in two independent samples. Clean-up was not necessary. Standard addition
method (SA) and calibration curve with 5-bromine-4-hydroxy-3-methoxybenzaldehyde (5-BrV)
as an internal standard (IS) were employed in the quantification. 2,6-DCP contents did not show
significant differences between extracts and/or methods. Results showed that the concentration
range of sex pheromone per female were 2.03-2.27 ng mL-1 and 2.06-2.24 ng mL-1 for SA and
IS methods, respectively. The methods provide a specific, sensitive and reliable technique for
determining 2,6-DCP levels in ticks.
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Palavras-chave
Amblyomma cajennense, Tick sex pheromone, 2,6-dichlorophenol, GC/MS-SIM, Internal standard, Standard addition
Citação
GACHOKA, Kennedy K.; COSTA, Deomar P.; LOULY, Carla C. B.; FERREIRA, Lorena L.; BORGES, Lígia M. F.; FARIA, Lourival C.; FERRI, Pedro H. Comparison of two methods to quantify 2,6-Dichlorophenol from Tick Amblyomma cajennense by GC/MS-SIM. Journal of the Brazilian Chemical Society, Campinas, v. 21, n. 9, p. 1642-1647, 2010.