Perfil sérico da fosfatase alcalina após o reparo de defeitos osteocondrais com células mononucleares da medula óssea e proteína morfogenética óssea em coelhos
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Data
2011-12
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Resumo
In this study, serum alkaline phosphatase activity
was measured in response to the repair of osteochondral defects in twenty-four New Zealand rabbits. The
animals were divided into three groups: a control (GC), those treated with bone marrow mononuclear cells
(GCM) and those that received mononuclear cells with autologous bone morphogenetic protein (BMP + GCM).
After exposing the trochlear groove of the left stifl e joint, a wedge-shaped segment was removed. Later, the
defect was fi lled with an osteochondral autograft preserved in 98% glycerin. For the GC group, only the bone
graft was performed. For the GCM, in addition to the graft, 2x106 seed mononuclear cells were implanted. For
the GCM + BMP, the same number of cells, associated with 1μg of bone morphogenetic protein, were intraarticularly
administered. The osteoblastic response was measured by analyzing the serum alkaline phosphatase
on day 0 (preoperative) 3, 15, 30, and 45 after surgery, and by radiographic examinations. Analysis of variance
in randomized blocks, factorial and Tukey’s test (p = 0.05) were made. The overall mean GCM was superior to
the other groups and the highest rates were among the 15th and 45th days postoperatively. The discrepancy in
values between individuals of the same group casts doubts on the veracity of the test.
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Células-tronco, Consolidação de fraturas, Marcador ósseo, Bone marker, Fracture healing, Stem cells
Citação
SOUZA, Luiz Augusto de; OLIVEIRA, Benito Juarez Nunes Alves de; EURIDES, Duvaldo; GUIMARÃES, Ednaldo Carvalho; SILVA, Luiz Antônio Franco da; ALVES, Lorena Borges; ARRUDA, Ana Flávia Delben Pereira; DIAS, Taís Andrade. Perfil sérico da fosfatase alcalina após o reparo de defeitos osteocondrais com células mononucleares da medula óssea e proteína morfogenética óssea em coelhos. Biotemas, Florianópolis, v. 24, n. 4, p. 103-109, dez. 2011.