Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/13599
Tipo do documento: Artigo
Título: Glutathione for the freezing of cooled equine semen, using different protocols
Autor: Oliveira, Rodrigo Arruda de
Piersanti, Rachel L.
Wolf, Caroline Antoniazzi
Viu, Marco Antônio de Oliveira
Gambarini, Mara Lúcia
Abstract: The aim of this study was to evaluate the effect of in vitro addition of glutathione in four different concentrations compared to the control group, using two different freezing systems: controlled-rate (AS) and manual (MS) freezer. The parameters evaluated were motility, strength, plasmatic and acrosomal membrane integrity of spermatozoa from twelve stallions. Ejaculates from stallions were collected three times per week, during four weeks. Gel-free semen was diluted in skim milk extender and cooled (16ºC) for 24 h. After cooling, extended semen was centrifuged at 600 x g for 10 min. The supernatant was removed and sperm pellets were re-suspended using the freezing extender with different glutathione concentrations (0, 2.5 mM, 5 mM, 7.5 mM, and 10 mM). Samples were then packed into 0.5 ml straws, which were divided into two parts: one for cryopreservation in a controlled-rate freezer (AS) and the other for a manual system (MS). In this study, the control group showed higher motility and better membrane integrity within treatments for the AS (P < 0.05). Furthermore, when an MS group was evaluated, the 2.5 mM glutathione group demonstrated better preservation for total motility and plasmatic membrane integrity. However, concentrations higher than 2.5 mM were deleterious to the spermatozoa in both controlled and manual freezing systems.
Palavras-chave: Antioxidant
Frozen semen
Spermatozoa
Stallion
País: Brasil
Unidade acadêmica: Escola de Veterinária e Zootecnia - EVZ (RG)
Citação: OLIVEIRA, R. A.; PIERSANTI, R. L.; WOLF, C.; VIU, M. A. O.; GAMBARINI, M. L. Glutathione for the freezing of cooled equine semen, using different protocols. Animal Reproduction, Belo Horizonte, v. 11, n. 2, p. 104-109, Apt./June 2014.
Tipo de acesso: Acesso Aberto
URI: http://repositorio.bc.ufg.br/handle/ri/13599
Data de publicação: Jun-2014
Aparece nas coleções:EVZ - Artigos publicados em periódicos

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