Situação atual do banco de DNA de recursos genéticos animais no Brasil
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Data
2005
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Resumo
Ex situ conservation include the cryopreservation
of genetic material: semen, oocytes,
embryos, somatic cells and DNA (FAO, 1998).
Although, it is not possible to regenerate whole
animals from isolated DNA, it has been proved
that DNA banking was useful in population genetic
studies and epidemiological investigations. The
Animal Genetics Laboratory (AGL) at Embrapa
Genetic Resources and Biotechnology -
Cenargen, Brasília - DF, Brazil, works with genetic
characterization of animal populations that belong
to the Brazilian conservation program. At the
same time, a DNA Bank is being set up, which
already has samples from several animals
species. The DNA was extracted from
cryopreserved leukocyte pellets or buffy coats.
After extraction it was quantified and checked
for its integrity and quality. Each specimen
extracted was divided in at least two samples.
One of them was used for characterization
studies (-20°C) while the other was stored at -
80°C at the DNA bank. All animals that were
collected received a code number and were
catalogued in a data bank where all the available
information was registered. The refinement of
molecular biology techniques increase the
usefulness of banked material that will provide a
ready reservoir of valuable scientific information.
At this time, the AGL DNA bank has specimens
of several breeds of six domestic species. Most
of these breeds are in risk of extinction and are
involved in the Conservation Program of Embrapa/
Cenargen. DNA banking is proving to be useful
for characterization of domestic animal populations
that are in Brazilian conservation program.
In near future, it might be the method of choice
when many representatives of a breed are to be
stored.
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Banco de DNA, Genética da conservação, DNA Bank, Conservation genetics
Citação
EGITO, A. A.; ALBUQUERQUE, M. S. M.; CASTRO, S. T. R.; PAIVA, S. R.; MARQUES, J. R. F.;McMANUS, C. M.;MARIANTE, A. S.; ABREU, U. G. P.; SANTOS, S. A.; SERENO, J. R. B.; FIORAVANTI, M. C. S.; VAZ, C. M.; NOBRE, F. V.; CARVALHO, J. H.; COSTA, M. R.; RIBEIRO, M. N.; LARA, M. A. C. Situação atual do banco de DNA de recursos genéticos animais no Brasil. Archivos de Zootecnia, Córdoba, v. 54, n. 206-207, p. 283-288, 2005.