Cellular immune response of Curraleiro Pé-duro and Nellore calves following Mycobacterium bovis-BCG vaccination
Carregando...
Data
2013-12
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
The present study aimed to assess the CD4, CD8 and gd blood levels for Curraleiro Pé-
-duro, as well as the specific IFN-g response after BCG vaccination using flow cytometry.
The specific immune response against BCG was also evaluated by tuberculin skin test, performed
before and 45 days after the vaccination. For comparison purposes, the same parameters
were investigated on Nellore calves, an exotic bovine with resistance previously
demonstrated. Naturally, Curraleiro Pé-duro animals had greater levels of CD4, CD8 and γδ
lymphocytes (p<0.05). In response to vaccine, Curraleiro Pé-duro showed greater ability
to respond specifically to BCG, generating resistance profile (Th1), evidenced by greater
number of antigen specific CD4+ cells producing IFN-γ (p<0.05) and also higher tuberculin
skin test reaction (p<0.05). Additionally, vaccinated Curraleiro Pé-duro calves had higher
CD4 cells numbers than both Nellore control (p<0.05) and vaccinated groups (p<0.05).
Curraleiro Pé-duro calves’ higher basal lymphocytes blood level and stronger response in
both IFN-γ and tuberculin skin test parameters probably play a positive role on protection/
resistance to Mycobacterium bovis.
Descrição
Palavras-chave
Mycobacterium bovis-BCG vaccination, Bovine resistance, Tuberculosis, Flow cytometry, Vacinação com Mycobacterium bovis- -BCG, Resistência bovina, Tuberculose bovina, Citometria de fluxo
Citação
MAGGIOLI, Mayara Fernanda; LOBO, Joyce Rodrigues; FIORAVANTI, Maria Clorinda Soares; KIPNIS, André; JUNQUEIRA-KIPNIS, Ana Paula. Cellular immune response of Curraleiro Pé-duro and Nellore calves following Mycobacterium bovis-BCG vaccination. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 33, n. 12, p. 1403-1408, Dec. 2013.