Imunofenotipagem e avaliação quantitativa de linfócitos circulantes de bovinos da raça Curraleiro
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Data
2009-04
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Resumo
The aim of this study was to establish an immunological profile by marking and
quantification of T and B lymphocytes in Curraleiro breed by immunocytochemistry. A total
of 116 cattle was used, males and females of different ages, from two farms situated in the
Goiás State, Brazil. The animals were allotted into groups according to age, sex and origin.
Blood samples were collected and processed in accordance with immunocytochemistry
standard technique using lymphoid markers species-specific, as anti-CD3 (MM1A - BoCD3)
and anti-LB (LCTB16A-clone B-B14), for T and B lymphocyte counting, respectively. The
data were submitted to descriptive statistics, and to Kruskall Wallis and Mann-Whitney
tests. The results showed decreased levels of leukocytes, lymphocytes, T lymphocytes
and B lymphocytes along the age advance. Absolute values of leukocytes, lymphocytes
and T lymphocytes were higher in males than females. None of the evaluated parameters
were affected by differences of the management carried out at the two farms.
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Palavras-chave
Imunocitoquímica, CD3, Linfócitos T, Linfócitos B, Perfil imunológico, Immunocytochemistry, T lymphocytes, B lymphocytes, Immunological profile
Citação
MORAES, Júlia M.; BRITO, Luiz A. B.; MOURA, Veridiana M. B. D. de; RIBEIRO, Carolina S.; GUIMARÃES, Victor Y.; ANDRADE, Diogo F.; LOBO, Joyce R.; FIORAVANTI, Maria Clorinda S. Imunofenotipagem e avaliação quantitativa de linfócitos circulantes de bovinos da raça Curraleiro. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 29, n. 4, p. 339-344, abr. 2009.