Embryonic stem-like cells derived from in vitro produced bovine blastocysts
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Data
2011-06
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Resumo
The aim of this work was to study the derivation of bovine embryonic stem-like (ES-like) cells from the inner cell
mass (ICM) of in vitro produced blastocysts. The ICMs were mechanically isolated and six out of seventeen (35%)
ICMs could attach to a monolayer of murine embryonic fibroblasts (MEF).Ten days after, primary outgrowths were
mechanically dissected into several small clumps and transferred to a new MEF layer. Cells were further
propagated and passaged by physical dissociation over a 60 days period. The pluripotency of the bovine ES-like
cells was confirmed by RT-PCR of Oct-4 and STAT-3 gene markers. The colonies were weakly stained for alkaline
phosphatase and the mesoderm and endoderm differentiation gene markers such as GATA-4 and Flk-1, respectively,
were not expressed. Embryoid bodies were spontaneously formed at the seventh passage. Results showed that
bovine ES-like cells could be obtained and passaged by mechanical procedures from the fresh in vitro produced
blastocysts.
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Bovine blastocysts, ICM isolation, Embryonic stem cells, Gene markers
Citação
FREITAS, Érika Regina Leal de; SANCHES, Bruno Valente; GAMBARINI, Maria Lúcia; OLIVEIRA FILHO, Benedito Dias; GUILLO, Lidia Andreu. Embryonic stem-like cells derived from in vitro produced bovine blastocysts. Brazilian Archives of Biology and Technology, Curitiba, v. 54, n. 3, p. 495-502, May/June 2011.