Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/13650
Tipo do documento: Artigo
Título: Embryonic stem-like cells derived from in vitro produced bovine blastocysts
Autor: Freitas, Érika Regina Leal de
Sanches, Bruno Valente
Gambarini, Maria Lúcia
Oliveira Filho, Benedito Dias
Guillo, Lidia Andreu
Abstract: The aim of this work was to study the derivation of bovine embryonic stem-like (ES-like) cells from the inner cell mass (ICM) of in vitro produced blastocysts. The ICMs were mechanically isolated and six out of seventeen (35%) ICMs could attach to a monolayer of murine embryonic fibroblasts (MEF).Ten days after, primary outgrowths were mechanically dissected into several small clumps and transferred to a new MEF layer. Cells were further propagated and passaged by physical dissociation over a 60 days period. The pluripotency of the bovine ES-like cells was confirmed by RT-PCR of Oct-4 and STAT-3 gene markers. The colonies were weakly stained for alkaline phosphatase and the mesoderm and endoderm differentiation gene markers such as GATA-4 and Flk-1, respectively, were not expressed. Embryoid bodies were spontaneously formed at the seventh passage. Results showed that bovine ES-like cells could be obtained and passaged by mechanical procedures from the fresh in vitro produced blastocysts.
Palavras-chave: Bovine blastocysts
ICM isolation
Embryonic stem cells
Gene markers
País: Brasil
Unidade acadêmica: Escola de Veterinária e Zootecnia - EVZ (RG)
Citação: FREITAS, Érika Regina Leal de; SANCHES, Bruno Valente; GAMBARINI, Maria Lúcia; OLIVEIRA FILHO, Benedito Dias; GUILLO, Lidia Andreu. Embryonic stem-like cells derived from in vitro produced bovine blastocysts. Brazilian Archives of Biology and Technology, Curitiba, v. 54, n. 3, p. 495-502, May/June 2011.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1590/S1516-89132011000300009
Identificador do documento: 10.1590/S1516-89132011000300009
URI: http://repositorio.bc.ufg.br/handle/ri/13650
Data de publicação: Jun-2011
Aparece nas coleções:EVZ - Artigos publicados em periódicos

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