Histopathological, immunohistochemical, and molecular study of BHV-5 infection in the central nervous system of experimentally infected calves
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Data
2015-04
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Resumo
Bovine meningoencephalitis caused by BHV-5, a double-stranded DNA enveloped virus
that belongs to the family Herpesviridae and subfamily Alphaherpesvirinae, is an important
differential diagnosis of central nervous diseases. The aim of this study was to describe the
histological changes in the central nervous system of calves experimentally infected with
BHV-5 and compare these changes with the PCR and IHC results. Formalin-fixed paraffin-
-embedded central nervous system samples from calves previously inoculated with BHV-5
were microscopically evaluated and tested using IHC and PCR. All the animals presented
with nonsuppurative meningoencephalitis. From 18 evaluated areas of each calf, 32.41%
and 35.19% were positive by IHC and PCR, respectively. The telencephalon presented more
accentuated lesions and positive areas in the PCR than other encephalic areas and was the
best sampling area for diagnostic purposes. Positive areas in the IHC and PCR were more
injured than IHC and PCR negative areas. The animal with neurological signs showed more
PCR- and IHC-positive areas than the other animals.
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Palavras-chave
Herpesvirus bovino 5, Doenças de bovinos, Meningoencefalite, Neuropatologia, Bovine herpesvirus 5, Diseases of cattle, Meningoencephalitis, Neuropathology
Citação
CAGNINI, Didier Q.; CUNHA, Paulo H. J.; PANTOJA, José C. F.; BADIAL, Peres R.; OLIVEIRA FILHO, José P.; ARAÚJO JÚNIOR, João P.; ALFIERI, Amauri A.; BORGES, Alexandre S. Histopathological, immunohistochemical, and molecular study of BHV-5 infection in the central nervous system of experimentally infected calves. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 35, n. 4, p. 337-343, Apr. 2015.