Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/14060
Tipo do documento: Artigo
Título: The Trypanosoma cruzi Satellite DNA OligoC-TesT and Trypanosoma cruzi Kinetoplast DNA OligoC-TesT for diagnosis of Chagas disease: a multi-cohort comparative evaluation study
Autor: De Winne, Koen
Buscher, Philippe
Ostermayer, Alejandro Luquetti
Tavares, Suelene Brito do Nascimento
Oliveira, Rodrigo A.
Solari, Aldo
Zulantay, Ines
Apt, Werner
Diosque, Patricio
Rumi, Mercedes Monje
Gironès, Nuria
Fresno, Manuel
Lopez-Velez, Rogelio
Perez-Molina, José Antônio
Monge-Maillo, Begoña
Garcia, Lineth
Deborggraeve, Stijn
Resumo: Background: The Trypanosoma cruzi satellite DNA (satDNA) OligoC-TesT is a standardised PCR format for diagnosis of Chagas disease. The sensitivity of the test is lower for discrete typing unit (DTU) TcI than for TcII-VI and the test has not been evaluated in chronic Chagas disease patients. Methodology/Principal Findings: We developed a new prototype of the OligoC-TesT based on kinetoplast DNA (kDNA) detection. We evaluated the satDNA and kDNA OligoC-TesTs in a multi-cohort study with 187 chronic Chagas patients and 88 healthy endemic controls recruited in Argentina, Chile and Spain and 26 diseased non-endemic controls from D.R. Congo and Sudan. All specimens were tested in duplicate. The overall specificity in the controls was 99.1% (95% CI 95.2%–99.8%) for the satDNA OligoC-TesT and 97.4% (95% CI 92.6%–99.1%) for the kDNA OligoC-TesT. The overall sensitivity in the patients was 67.9% (95% CI 60.9%–74.2%) for the satDNA OligoC-TesT and 79.1% (95% CI 72.8%–84.4%) for the kDNA OligoC-Test. Conclusions/Significance: Specificities of the two T. cruzi OligoC-TesT prototypes are high on non-endemic and endemic controls. Sensitivities are moderate but significantly (p = 0.0004) higher for the kDNA OligoC-TesT compared to the satDNA OligoC-TesT.
Palavras-chave: Trypanosoma cruzi
Chagas disease
Diagnosis of Chagas disease
País: Estados unidos
Unidade acadêmica: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
Citação: DE WINNE, Koen; BÜSCHER, Philippe; LUQUETTI, Alejandro O.; TAVARES, Suelene B. N.; OLIVEIRA, Rodrigo A.; SOLARI, Aldo; ZULANTAY, Ines; APT, Werner; DIOSQUE, Patricio; RUMI, Mercedes Monje; GIRONÈS, Nuria; FRESNO, Manuel; LOPEZ-VELEZ, Rogelio; PEREZ-MOLINA, José A.; MONGE-MAILLO, Begoña; GARCIA, Lineth; DEBORGGRAEVE, Stijn. The Trypanosoma cruzi Satellite DNA OligoC-TesT and Trypanosoma cruzi Kinetoplast DNA OligoC-TesT for diagnosis of Chagas disease: a multi-cohort comparative evaluation study. PLoS Neglected Tropical Diseases, San Francisco, v. 8, n. 1, p. e2633, Jan. 2014.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1371/ journal.pntd.0002633
Identificador do documento: 10.1371/ journal.pntd.0002633
URI: http://repositorio.bc.ufg.br/handle/ri/14060
Data de publicação: Jan-2014
Aparece nas coleções:FM - Artigos publicados em periódicos

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