Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/14076
Tipo do documento: Artigo
Título: Lipopolysaccharide infusion up-regulates hepcidin mRNA expression in equine liver
Autor: Oliveira Filho, José Paes
Badial, Peres Ramos
Cunha, Paulo Henrique Jorge da
Peiró, Juliana Regina
Araújo Júnior, João Pessoa
Divers, Thomas J.
Winand, Nena J.
Borges, Alexandre Secorun
Resumo: Hepcidin has been found to be the key regulator of iron metabolism that leads to hypoferremia during inflammation. Recent work has shown that equine hepcidin is predominantly expressed in the liver of horses. In this study, hepcidin gene expression was determined in the liver and bone marrow of six healthy horses after IV infusion of Escherichia coli O55:B5 LPS. The IL-6 gene expression was also determined in liver and bone marrow samples. Clinical and laboratory evaluations were measured at multiple time points between 0 and 240 h post-LPS infusion (PI). Liver and bone marrow biopsies were taken immediately before (baseline) and at 6 and 18 h PI. In response to endotoxin infusion, all horses showed characteristic clinical signs of endotoxemia. Plasma iron concentration was decreased significantly from the preinfusion level at 8 h PI. Hypoferremia peak was observed at 12 h and returned to normal levels at 30 h PI. Relative realtime RT-PCR analysis showed that liver hepcidin and IL-6 mRNA expression was up-regulated at 6 h PI. Bone marrow hepcidin relative expression was not influenced by LPS infusion. In another experiment, equine monocyte cultures were stimulated with LPS (1 mg/ml). Monocyte hepcidin and IL-6 gene expression was significantly induced after 2 h of LPS stimulus and returned to baseline levels thereafter. The present study describes that, in horses, LPS infusion up-regulates hepatic hepcidin mRNA expression resulting in early observed hypoferremia and suggests that hepcidin may act as an acute-phase protein in horses.
Palavras-chave: Acute-phase reactants
Endotoxins
Hepcidin
Inflammation
Iron metabolism
País: Gra-bretanha
Unidade acadêmica: Escola de Veterinária e Zootecnia - EVZ (RG)
Citação: OLIVEIRA FILHO, José P.; BADIAL, Peres R.; CUNHA, Paulo H. J.; PEIRÓ, Juliana R.; ARAÚJO JÚNIOR, João P.; DIVERS, Thomas J.; WINAND, Nena J.; BORGES, Alexandre S. Lipopolysaccharide infusion up-regulates hepcidin mRNA expression in equine liver. Innate Immunity, London, v. 18, n. 3, p. 438-446, 2011.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1177/1753425911420181
Identificador do documento: 10.1177/1753425911420181
URI: http://repositorio.bc.ufg.br/handle/ri/14076
Data de publicação: 2011
Aparece nas coleções:EVZ - Artigos publicados em periódicos

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