Evaluation of solid-phase microextraction using a polythiophene film and liquid chromatography with spectrophotometric detection for the determination of antidepressants in plasma samples
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Data
2012
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Resumo
Polythiophene (PTh) phase electropolymerized on the stainless steel wire was evaluated as solidphase
microextraction (SPME), and analysis by liquid chromatography with spectrophotometric
detection (LC-UV) for determination of new-generation antidepressants, selective serotonin
reuptake inhibitors (SSRIs) (citalopram, paroxetine, fluoxetine and sertraline), in plasma samples.
The influence of electropolymerization variables (scan rate, potential range and scan cycles) was
evaluated on SPME performance. The SPME variables (extraction time, temperature, matrix pH,
ionic strength and desorption procedure), as well as the influence of plasma proteins on sorption
mechanisms were also evaluated. The SPME/LC-UV method developed for determination of
antidepressants in plasma sample presented a linear range between the limit of quantification
(LOQ, 200-250 ng mL-1) to 4000 ng mL-1, and interday precision with coefficient of variation
(CV) ranged from 11 to 15%. The proposed method can be a useful tool for the determination of
antidepressants in human plasma samples in urgent toxicological analysis after the accidental or
suicidal intake of higher doses of medications.
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Solid-phase microextraction, Polythiophene, Antidepressants, Liquid chromatography
Citação
CARIS, Juciene A. ; CHAVES, AndrĂ©a R.; QUEIROZ, Maria EugĂȘnia C. Evaluation of solid-phase microextraction using a polythiophene film and liquid chromatography with spectrophotometric detection for the determination of antidepressants in plasma samples. Journal of the Brazilian Chemical Society, Campinas, v. 23, n. 1, p. 57-64, 2012.