Toxicity of cryoprotectants on Prochilodus lineatus (Valenciennes, 1837) (curimba) embryos in an experimental incubator (Characiformes: Prochilodontidae)
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2014-12
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This paper investigated the effect of cryoprotectant substances on Prochilodus lineatus embryos in an experimental
incubator. The prospective study applied combinations of polyvinyl alcohol, hydroxyethyl cellulose, gelatin and fetal bovine
serum with dimethyl sulfoxide and ethylene glycol in a new experimental incubator. The morphology of embryos, larval
viability and the efficiency of experimental incubators in maintaining the quality of embryos were evaluated. This study
demonstrates the efficient association between hydroxyethylcellulose and dimethyl sulfoxide as greater viability (p<0.05)
was found for embryos (72.9 ± 23.9%). It should also be noted the permeation of cryoprotectants in embryos through the
changes found in chorion diameter, embryo diameter and embryo volume comparing the treatments versus control group
(water) (p<0.05), this results can help in future cryopreservation protocols. Although the temperature and oxygenation
differed between the usual and experimental incubators (p<0.05), the results showed a high fertilization rate (79.6 ± 13.2%)
for experimental incubators (p<0.05) which is sufficient for the maintenance of embryos in a cryoprotective environment
and effectively allows experimentation for long periods with cryoprotectant substances. Cryopreservation of fish embryos
has not been accomplished yet and new approaches are required for understanding the permeability of teleost embryos,
especially in Brazilian native species.
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Fertilization, Freshwater fish, Morphology, Reproduction
Citação
PAULA, Daniella A. J. et al. Toxicity of cryoprotectants on Prochilodus lineatus (Valenciennes, 1837) (curimba) embryos in an experimental incubator (Characiformes: Prochilodontidae). Neotropical Ichthyology, Porto Alegre, v. 12, n. 4, p. 835-844, Oct./Dec. 2014.