Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/15343
Tipo do documento: Artigo
Título: Distinct chitinases are expressed during various growth phases of the human pathogen Paracoccidioides brasiliensis
Autor: Santana, Lidiane Aparecida da Penha
Vainstein, Marilene Henning
Tomazett, Patrícia Kott
Silva, Ludier Kesser Santos
Góes, Alfredo Miranda
Schrank, Augusto
Soares, Célia Maria de Almeida
Pereira, Maristela
Abstract: The aim of this work was the partial purification and subsequent evaluation of chitinase expression during the various growth phases of Paracoccidioides brasiliensis. Initially, PbCTS1r was expressed as a recombinant pro- tein and displayed enzymatic activity against 4-MU-[N-acetylglucosamine (GlcNAc)] 3 and 4-MU-(GlcNAc) 2 . Two proteins, 45 kDa and 39 kDa in size, were partially purified from P. brasiliensis yeast crude extract using cation- exchange chromatography coupled with HPLC and were characterised as PbCTS1 and PbCTS2, respectively. Anti- PbCTS1r antibody recognised two proteins in the crude extracts of yeast and the transitional stage between mycelial and yeast phases. In crude extracts of mycelium, only the 45 kDa protein was detected. However, quantitative real- time polymerase chain reaction led to the detection of small quantities of Pbcts2 transcript in the mycelial phase. In the yeast cell wall extract, only the 39 kDa protein was detected. Moreover, both proteins were secreted by the yeast parasitic phase, suggesting that these proteins participate in the modulation of the fungal environment. Phylogenetic analysis of the predicted PbCTS1 and PbCTS2 proteins indicated that they code for distinct chitinases in P. brasilien- sis. During evolution, P. brasiliensis could have acquired the paralogues Pbcts1 and Pbcts2 for growth and survival in diverse environments in both saprophytic and parasitic phases.
Palavras-chave: Paracoccidioides
Cell wall
Chitinase
Chromatography
País: Brasil
Unidade acadêmica: Instituto de Ciências Biológicas - ICB (RG)
Citação: SANTANA, Lidiane Aparecida da Penha; VAINSTEIN, Marilene Henning; TOMAZETT, Patrícia Kott; SANTOS-SILVA, Ludier Kesser; GÓES, Alfredo Miranda; SCHRANK, Augusto; SOARES, Célia Maria de Almeida; PEREIRA, Maristela. Distinct chitinases are expressed during various growth phases of the human pathogen Paracoccidioides brasiliensis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 107, n. 3, p. 310-316, May 2012.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1590/S0074-02762012000300004
Identificador do documento: 10.1590/S0074-02762012000300004
URI: http://repositorio.bc.ufg.br/handle/ri/15343
Data de publicação: Mai-2012
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

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