Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/15406
Tipo do documento: Artigo
Título: Heterologous expression, purification and immunological reactivity of a recombinant HSP60 from Paracoccidioides brasiliensis
Autor: Cunha, Daniela A.
Oliveira, Rosely Maria Zancopé
Felipe, Maria Sueli Soares
Furlaneto, Silvia Maria Salem Izacc
Deepe Junior, George S.
Soares, Célia Maria de Almeida
Abstract: The complete coding cDNA of HSP60 from Paracoccidioides brasiliensis was overexpressed in an Escherichia coli host to produce high levels of recombinant protein. The protein was purified by affinity chromatography. A total of 169 human serum samples were tested for reactivity by Western blot analysis with the purified HSP60 recombinant protein. Immunoblots indicated that the recombinant P. brasiliensis HSP60 was recognized by antibodies in 72 of 75 sera from paracoccidioidomycosis patients. No cross-reactivity was detected with individual sera from patients with aspergillosis, sporotrichosis, cryptococcosis, and tuberculosis. Reactivity to HSP60 was observed in sera from 9.52% of control healthy individuals and 11.5% of patients with histoplas- mosis. The high sensitivity and specificity (97.3 and 92.5%, respectively) for HSP60 suggested that the recombinant protein can be used singly or in association with other recombinant antigens to detect antibody responses in P. brasiliensis-infected patients.
País: Estados unidos
Unidade acadêmica: Instituto de Ciências Biológicas - ICB (RG)
Citação: CUNHA, Daniela A.; ZANCOPÉ-OLIVEIRA, Roseli M.; FELIPE, M. Sueli S.; SALEM-IZACC, Silvia M.; DEEPE JR, George S ; SOARES, Célia M. A. Heterologous expression, purification and immunological reactivity of a recombinant HSP60 from Paracoccidioides brasiliensis. Clinical and Diagnostic Laboratory Immunology, Washington, v. 9, n. 2, p. 374-377, Mar. 2002.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1128/CDLI.9.2.374–377.2002
Identificador do documento: 10.1128/CDLI.9.2.374–377.2002
URI: http://repositorio.bc.ufg.br/handle/ri/15406
Data de publicação: Mar-2002
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

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