Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/15407
Tipo do documento: Artigo
Título: Anti-CEA loaded maghemite nanoparticles as a theragnostic device for colorectal cancer
Autor: Paz, Mariana Campos da
Santos, Maria de Fátima M. Almeida
Santos, Camila M.B.
Silva, Sebastiao William da
Souza, Lincoln Bernardo de
Lima, Emilia Celma de Oliveira
Silva, Renata C.
Lucci, Carolina Madeira
Morais, Paulo César
Azevedo, Ricardo B.
Lacava, Zulmira Guerrero Marques
Abstract: Nanosized maghemite particles were synthesized, precoated (with dimercaptosuccinic acid) and surface-functionalized with anticarcinoembryonic antigen (anti-CEA) and successfully used to target cell lines expressing the CEA, characteristic of colorectal cancer (CRC) cells. The as-developed nanosized material device, consisting of surface decorated maghemite nanoparticles suspended as a biocompatible magnetic fluid (MF) sample, labeled MF-anti-CEA, was characterized and tested against two cell lines: a high-CEA expressing cell line (LS174T) and a low-CEA expressing cell line (HCT116). Whereas X-ray diffraction was used to assess the average core size of the as-synthesized maghemite particles (average 8.3 nm in diameter), dynamic light scattering and electrophoretic mobility measurements were used to obtain the average hydrodynamic diameter (550 nm) and the zeta-potential (‑38 mV) of the as-prepared and maghemite-based nanosized device, respectively. Additionally, surface-enhanced Raman spectroscopy (SERS) was used to track the surface decoration of the nanosized maghemite particles from the very first precoating up to the attachment of the anti-CEA moiety. The Raman peak at 1655 cm‑1, absent in the free anti-CEA spectrum, is the signature of the anti-CEA binding onto the precoated magnetic nanoparticles. Whereas MTT assay was used to confirm the low cell toxicity of the MF-anti-CEA device, ELISA and Prussian blue iron staining tests performed with both cell lines (LS174T and HCT116) confirm that the as-prepared MF-anti-CEA is highly specific for CEA-expressing cells. Finally, transmission electron microscopy analyses show that the association with anti-CEA seems to increase the number of LS174T cells with internalized maghemite nanoparticles, whereas no such increase seems to occur in the HCT116 cell line. In conclusion, the MF-anti-CEA sample is a biocompatible device that can specifically target CEA, suggesting its potential use as a theragnostic tool for CEA-expressing tumors, micrometastasis, and cancer-circulating cells.
Palavras-chave: Magnetic nanoparticles
Anti-CEA antibody
Targeted delivery
Diagnostic
Raman
Biocompatible device
País: Gra-bretanha
Unidade acadêmica: Instituto de Química - IQ (RG)
Citação: PAZ, Mariana Campos da et al. Anti-CEA loaded maghemite nanoparticles as a theragnostic device for colorectal cancer. International Journal of Nanomedicine, Macclesfield, v. 7, p. 5271-5282, Oct. 2012.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.2147/IJN.S32139
Identificador do documento: 10.2147/IJN.S32139
URI: http://repositorio.bc.ufg.br/handle/ri/15407
Data de publicação: Out-2012
Aparece nas coleções:IQ - Artigos publicados em periódicos

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