Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/15645
Tipo do documento: Artigo
Título: Down-regulation of CTLA-4 by HIV-1 nef protein
Autor: El Far, Mohamed
Isabelle, Catherine
Chomont, Nicolas
Bourbonnière, Martin
Fonseca, Simone Gonçalves da
Ancuta, Petronela
Peretz, Yoav
Chouikh, Younes
Halwani, Rabih
Schwartz, Olivier
Madrenas, Joaquı́n
Freeman, Gordon J.
Routy, Jean-Pierre
Haddad, Elias K.
Sékaly, Rafick-Pierre
Abstract: HIV-1 Nef protein down-regulates several cell surface receptors through its interference with the cell sorting and trafficking machinery. Here we demonstrate for the first time the ability of Nef to down-regulate cell surface expression of the negative immune modulator CTLA-4. Down-regulation of CTLA-4 required the Nef motifs DD175, EE155 and LL165, all known to be involved in vesicle trafficking. Disruption of the lysosomal functions by pH-neutralizing agents prevented CTLA-4 down- regulation by Nef, demonstrating the implication of the endosomal/lysosomal compartments in this process. Confocal microscopy experiments visualized the co-localization between Nef and CTLA-4 in the early and recycling endosomes but not at the cell surface. Overall, our results provide a novel mechanism by which HIV-1 Nef interferes with the surface expression of the negative regulator of T cell activation CTLA-4. Down-regulation of CTLA-4 may contribute to the mechanisms by which HIV-1 sustains T cell activation, a critical step in viral replication and dissemination.
País: Estados unidos
Unidade acadêmica: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
Citação: EL-FAR, Mohamed; ISABELLE, Catherine; CHOMONT, Nicolas; BOURBONNIÈRE, Martin; FONSECA, Simone; ANCUTA, Petronela; PERETZ, Yoav; CHOUIKH,Younes; HALWANI, Rabih; SCHWARTZ, Olivier; MADRENAS, Joaquín; FREEMAN, Gordon J.; ROUTY, Jean-Pierre; HADDAD, Elias K.; SÉKALY, Rafick-Pierre. Down-regulation of CTLA-4 by HIV-1 nef protein. Plos One, San Francisco, v. 8, n. 1, p. e54295, Jan. 2013.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1371/journal.pone.0054295
Identificador do documento: 10.1371/journal.pone.0054295
URI: http://repositorio.bc.ufg.br/handle/ri/15645
Data de publicação: Jan-2013
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

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